Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila.

N. J. Maihle, Birgit H. Satir

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryotic cell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.

Original languageEnglish (US)
Pages (from-to)195-202
Number of pages8
JournalJournal of Cellular Biochemistry
Volume31
Issue number3
StatePublished - 1986

Fingerprint

Tetrahymena thermophila
Secretory Pathway
Biomarkers
Tetrahymena
Peptides
Eukaryotic Cells
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Optical microscopy
Immune Sera
Carrier Proteins
Proteins
Research Personnel
Light

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila. / Maihle, N. J.; Satir, Birgit H.

In: Journal of Cellular Biochemistry, Vol. 31, No. 3, 1986, p. 195-202.

Research output: Contribution to journalArticle

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