Abstract
Condensin is a central regulator of mitotic genome structure with mutants showing poorly condensed chromosomes and profound segregation defects. Here, we identify NCT, a complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), and several TAFs, as a regulator of condensin function. We show that NCT and condensin bind similar genomicregions but only briefly colocalize during the periods of chromosome condensation and decondensation. This pattern of NCT binding at the core centromere, the region of maximal condensin enrichment, tracks the abundance of acetylated histone H4, as regulated by the Hat1-Mis16 acetyltransferase complex and recognized by thefirst Nrc1 bromodomain. Strikingly, mutants inNCT or Hat1-Mis16 restore the formation of segregation-competent chromosomes in cells containing defective condensin. These results are consistent with a model where NCT targets CKII to chromatin in a cell-cycle-directed manner inorder to modulate the activity of condensin duringchromosome condensation and decondensation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 892-905 |
| Number of pages | 14 |
| Journal | Cell Reports |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2014 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensin function. / Kim, Hyun Soo; Mukhopadhyay, Rituparna; Rothbart, Scott B.; Silva, Andrea C.; Vanoosthuyse, Vincent; Radovani, Ernest; Kislinger, Thomas; Roguev, Assen; Ryan, Colm J.; Xu, Jiewei; Jahari, Harlizawati; Hardwick, Kevin G.; Greenblatt, Jack F.; Krogan, Nevan J.; Fillingham, Jeffrey S.; Strahl, Brian D.; Bouhassira, Eric E.; Edelmann, Winfried; Keogh, Michael Christopher.
In: Cell Reports, Vol. 6, No. 5, 2014, p. 892-905.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensin function
AU - Kim, Hyun Soo
AU - Mukhopadhyay, Rituparna
AU - Rothbart, Scott B.
AU - Silva, Andrea C.
AU - Vanoosthuyse, Vincent
AU - Radovani, Ernest
AU - Kislinger, Thomas
AU - Roguev, Assen
AU - Ryan, Colm J.
AU - Xu, Jiewei
AU - Jahari, Harlizawati
AU - Hardwick, Kevin G.
AU - Greenblatt, Jack F.
AU - Krogan, Nevan J.
AU - Fillingham, Jeffrey S.
AU - Strahl, Brian D.
AU - Bouhassira, Eric E.
AU - Edelmann, Winfried
AU - Keogh, Michael Christopher
PY - 2014
Y1 - 2014
N2 - Condensin is a central regulator of mitotic genome structure with mutants showing poorly condensed chromosomes and profound segregation defects. Here, we identify NCT, a complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), and several TAFs, as a regulator of condensin function. We show that NCT and condensin bind similar genomicregions but only briefly colocalize during the periods of chromosome condensation and decondensation. This pattern of NCT binding at the core centromere, the region of maximal condensin enrichment, tracks the abundance of acetylated histone H4, as regulated by the Hat1-Mis16 acetyltransferase complex and recognized by thefirst Nrc1 bromodomain. Strikingly, mutants inNCT or Hat1-Mis16 restore the formation of segregation-competent chromosomes in cells containing defective condensin. These results are consistent with a model where NCT targets CKII to chromatin in a cell-cycle-directed manner inorder to modulate the activity of condensin duringchromosome condensation and decondensation.
AB - Condensin is a central regulator of mitotic genome structure with mutants showing poorly condensed chromosomes and profound segregation defects. Here, we identify NCT, a complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), and several TAFs, as a regulator of condensin function. We show that NCT and condensin bind similar genomicregions but only briefly colocalize during the periods of chromosome condensation and decondensation. This pattern of NCT binding at the core centromere, the region of maximal condensin enrichment, tracks the abundance of acetylated histone H4, as regulated by the Hat1-Mis16 acetyltransferase complex and recognized by thefirst Nrc1 bromodomain. Strikingly, mutants inNCT or Hat1-Mis16 restore the formation of segregation-competent chromosomes in cells containing defective condensin. These results are consistent with a model where NCT targets CKII to chromatin in a cell-cycle-directed manner inorder to modulate the activity of condensin duringchromosome condensation and decondensation.
UR - http://www.scopus.com/inward/record.url?scp=84895919564&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84895919564&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2014.01.029
DO - 10.1016/j.celrep.2014.01.029
M3 - Article
C2 - 24565511
AN - SCOPUS:84895919564
VL - 6
SP - 892
EP - 905
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 5
ER -