Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensin function

Hyun Soo Kim, Rituparna Mukhopadhyay, Scott B. Rothbart, Andrea C. Silva, Vincent Vanoosthuyse, Ernest Radovani, Thomas Kislinger, Assen Roguev, Colm J. Ryan, Jiewei Xu, Harlizawati Jahari, Kevin G. Hardwick, Jack F. Greenblatt, Nevan J. Krogan, Jeffrey S. Fillingham, Brian D. Strahl, Eric E. Bouhassira, Winfried Edelmann, Michael Christopher Keogh

Research output: Contribution to journalArticle

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Abstract

Condensin is a central regulator of mitotic genome structure with mutants showing poorly condensed chromosomes and profound segregation defects. Here, we identify NCT, a complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), and several TAFs, as a regulator of condensin function. We show that NCT and condensin bind similar genomicregions but only briefly colocalize during the periods of chromosome condensation and decondensation. This pattern of NCT binding at the core centromere, the region of maximal condensin enrichment, tracks the abundance of acetylated histone H4, as regulated by the Hat1-Mis16 acetyltransferase complex and recognized by thefirst Nrc1 bromodomain. Strikingly, mutants inNCT or Hat1-Mis16 restore the formation of segregation-competent chromosomes in cells containing defective condensin. These results are consistent with a model where NCT targets CKII to chromatin in a cell-cycle-directed manner inorder to modulate the activity of condensin duringchromosome condensation and decondensation.

Original languageEnglish (US)
Pages (from-to)892-905
Number of pages14
JournalCell Reports
Volume6
Issue number5
DOIs
StatePublished - 2014

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Casein Kinase II
Chromosomes
Chromosome Segregation
Condensation
Acetyltransferases
Centromere
Histones
Chromatin
condensin complexes
Cell Cycle
Genes
Cells
Genome
Defects
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kim, H. S., Mukhopadhyay, R., Rothbart, S. B., Silva, A. C., Vanoosthuyse, V., Radovani, E., ... Keogh, M. C. (2014). Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensin function. Cell Reports, 6(5), 892-905. https://doi.org/10.1016/j.celrep.2014.01.029

Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensin function. / Kim, Hyun Soo; Mukhopadhyay, Rituparna; Rothbart, Scott B.; Silva, Andrea C.; Vanoosthuyse, Vincent; Radovani, Ernest; Kislinger, Thomas; Roguev, Assen; Ryan, Colm J.; Xu, Jiewei; Jahari, Harlizawati; Hardwick, Kevin G.; Greenblatt, Jack F.; Krogan, Nevan J.; Fillingham, Jeffrey S.; Strahl, Brian D.; Bouhassira, Eric E.; Edelmann, Winfried; Keogh, Michael Christopher.

In: Cell Reports, Vol. 6, No. 5, 2014, p. 892-905.

Research output: Contribution to journalArticle

Kim, HS, Mukhopadhyay, R, Rothbart, SB, Silva, AC, Vanoosthuyse, V, Radovani, E, Kislinger, T, Roguev, A, Ryan, CJ, Xu, J, Jahari, H, Hardwick, KG, Greenblatt, JF, Krogan, NJ, Fillingham, JS, Strahl, BD, Bouhassira, EE, Edelmann, W & Keogh, MC 2014, 'Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensin function', Cell Reports, vol. 6, no. 5, pp. 892-905. https://doi.org/10.1016/j.celrep.2014.01.029
Kim, Hyun Soo ; Mukhopadhyay, Rituparna ; Rothbart, Scott B. ; Silva, Andrea C. ; Vanoosthuyse, Vincent ; Radovani, Ernest ; Kislinger, Thomas ; Roguev, Assen ; Ryan, Colm J. ; Xu, Jiewei ; Jahari, Harlizawati ; Hardwick, Kevin G. ; Greenblatt, Jack F. ; Krogan, Nevan J. ; Fillingham, Jeffrey S. ; Strahl, Brian D. ; Bouhassira, Eric E. ; Edelmann, Winfried ; Keogh, Michael Christopher. / Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensin function. In: Cell Reports. 2014 ; Vol. 6, No. 5. pp. 892-905.
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AU - Xu, Jiewei

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AU - Keogh, Michael Christopher

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