Identification and quantification of thymosin β4 in human saliva and tears

Mahnaz Badamchian, Ali A. Damavandy, Hadi Damavandy, Sonal D. Wadhwa, Barrett Katz, Allan L. Goldstein

Research output: Chapter in Book/Report/Conference proceedingConference contribution

33 Citations (Scopus)

Abstract

Thymosin β4 (Tβ4) is a ubiquitous, naturally occurring, 43-amino acid peptide that takes part in several biological activities including angiogenesis, inhibition of inflammation,wound healing, chemotaxis, and endothelial cell migration. Recent studies also indicate that Tβ4 accelerates corneal wound healing and downregulates several proinflamatory chemokines and cytokines. In this study, we sought to determine whether Tβ4 is naturally occurring in human tears and other human bodily fluids, such as saliva. Tear and saliva samples were analyzed by EIA to identify and quantify the amount of Tβ4 present. Around 10-20 samples were collected from each of three different age groups: 15-20, 25-35, and >50 years old with n = 30 and n = 60 for tears and saliva, respectively. Exclusion criteria included the use of any topical ophthalmic or topical oral medication and/or history of ocular or oral surgery within the past 6 months. Tears were collected from both eyes using Schirmer's strips. Saliva samples were collected in sterile tubes and were then centrifuged to remove solid particles. Tβ4 was found in tear and saliva samples in all age groups. The concentrations ranged from 0.5-7 μg/mL in tears and 0.2-3.6 μg/mL in saliva. In both fluids, Tβ4 concentration varied with age and appeared to peak at ages 25-35 years. Studies are in progress to determine if Tβ4 levels in saliva and tears demonstrate a circadian rhythm during a 24-h period, as well as to confirm that they vary with age and to explore if they vary with diseased states. This is the first study to report the presence of Tβ4 in human tears and saliva. This finding raises the possibility that Tβ4 acts as an endogenous agent contributing to the rapid healing of corneal and oralwounds. Considering that Tβ4 facilitates reepithelialization and modulates anti-inflammatory mediators, Tβ4 could potentially be used therapeutically in the treatment of (a) ocular surface disease and injury of eye and (b) various oral disorders, such as periodontal disease.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages458-465
Number of pages8
Volume1112
DOIs
StatePublished - Sep 2007
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1112
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Thymosin
Tears
Saliva
Wound Healing
Age Groups
Eye Injuries
Fluids
Eye Diseases
Oral Surgery
Endothelial cells
Periodontal Diseases
Chemotaxis
Circadian Rhythm
Bioactivity
Chemokines
Surgery
Cell Movement
Anti-Inflammatory Agents
Down-Regulation
Endothelial Cells

Keywords

  • Human saliva
  • Human tears
  • Thymosin β
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Badamchian, M., Damavandy, A. A., Damavandy, H., Wadhwa, S. D., Katz, B., & Goldstein, A. L. (2007). Identification and quantification of thymosin β4 in human saliva and tears. In Annals of the New York Academy of Sciences (Vol. 1112, pp. 458-465). (Annals of the New York Academy of Sciences; Vol. 1112). https://doi.org/10.1196/annals.1415.046

Identification and quantification of thymosin β4 in human saliva and tears. / Badamchian, Mahnaz; Damavandy, Ali A.; Damavandy, Hadi; Wadhwa, Sonal D.; Katz, Barrett; Goldstein, Allan L.

Annals of the New York Academy of Sciences. Vol. 1112 2007. p. 458-465 (Annals of the New York Academy of Sciences; Vol. 1112).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Badamchian, M, Damavandy, AA, Damavandy, H, Wadhwa, SD, Katz, B & Goldstein, AL 2007, Identification and quantification of thymosin β4 in human saliva and tears. in Annals of the New York Academy of Sciences. vol. 1112, Annals of the New York Academy of Sciences, vol. 1112, pp. 458-465. https://doi.org/10.1196/annals.1415.046
Badamchian M, Damavandy AA, Damavandy H, Wadhwa SD, Katz B, Goldstein AL. Identification and quantification of thymosin β4 in human saliva and tears. In Annals of the New York Academy of Sciences. Vol. 1112. 2007. p. 458-465. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1415.046
Badamchian, Mahnaz ; Damavandy, Ali A. ; Damavandy, Hadi ; Wadhwa, Sonal D. ; Katz, Barrett ; Goldstein, Allan L. / Identification and quantification of thymosin β4 in human saliva and tears. Annals of the New York Academy of Sciences. Vol. 1112 2007. pp. 458-465 (Annals of the New York Academy of Sciences).
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