Identical clonal origin of synchronous and metachronous low-grade, noninvasive papillary transitional cell carcinomas of the urinary tract

Li Maomi, Linda A. Cannizzaro

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

One of the most important biological features of papillary transitional cell carcinoma (pTCC) of the urinary tract is its multicentricity and its tendency for recurrences. Two possible mechanisms, field effect and intramucosal seeding/spreading, have been proposed. The former theory hypothesizes that carcinogenic agents cause synchronous or metachronous malignant transformation of multiple urothelial cells (independent clonal origin), and the latter speculates that synchronous and metachronous tumors are derived from implantation or direct spreading of tumor cells (identical clonal origin). We tested these hypotheses by analyzing the methylation patterns of the androgen receptor gene (HUMARA) located at the X-chromosome. Thirty-five metachronous and synchronous, lowgrade (grade 1 or 2), noninvasive pTCCs of the urinary tract from 10 heterozygous female patients were successfully analyzed using formalin-fixed, paraffin-embedded tissue. These included 16 recurrent bladder tumors from 4 patients, 10 metachronous bladder and ureter/renal pelvis tumors from 4 patients, and 9 multifocal tumors from 2 patients. All tumors are monoclonal as indicated by unbalanced methylation of HUMARA. Furthermore, same methylated allele was detected in multiple recurrent or multifocal tumors from any given patient, indicating their identical clonal origin. We conclude that low-grade, noninvasive pTCCs are monoclonal in nature. Synchronous or metachronous pTCCs have an identical clonal origin, strongly supporting the intramucosal seeding/spreading hypothesis.

Original languageEnglish (US)
Pages (from-to)1197-1200
Number of pages4
JournalHuman Pathology
Volume30
Issue number10
DOIs
StatePublished - 1999

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Transitional Cell Carcinoma
Papillary Carcinoma
Urinary Tract
Neoplasms
Methylation
Kidney Pelvis
Androgen Receptors
X Chromosome
Ureter
Urinary Bladder Neoplasms
Paraffin
Formaldehyde
Urinary Bladder
Alleles
Recurrence
Genes

Keywords

  • Field effect
  • Intramucosal seeding
  • Monoclonality
  • Papillary transitional cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Identical clonal origin of synchronous and metachronous low-grade, noninvasive papillary transitional cell carcinomas of the urinary tract. / Maomi, Li; Cannizzaro, Linda A.

In: Human Pathology, Vol. 30, No. 10, 1999, p. 1197-1200.

Research output: Contribution to journalArticle

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