Hypoxia markers in human osteosarcoma: An exploratory study

Hiroo Mizobuchi, José Manuel García-Castellano, Shaji Philip, John H. Healey, Richard Gorlick

Research output: Contribution to journalArticle

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Abstract

Neoplastic cells growing under hypoxic conditions exhibit a more aggressive phenotype by activating a cascade of molecular events partly mediated by hypoxia-inducible transcription factor (HIF-1α) and vascular endothelial growth factor (VEGF). The roles of these markers have been studied previously in several cancer lines. We ascertained the frequency of HIF-1α expression, VEGF expression, the degree of neovascularization, and cell proliferation in osteosarcoma samples. Samples from osteosarcoma patients were assessed for HIF-1α and VEGF protein expression using immunohistochemistry, neovascularization using antibodies for Factor VIII, and cell proliferation using the Ki-67 labeling index. Associations between these parameters and clinical features were examined. HIF-1α staining was positive in 35% of patients and metastases were present in 61% of these HIF-1α-positive patients. VEGF protein expression was detected in 69% of patients, 92% of whom were female. We observed an insignificant trend for a higher frequency of VEGF expression in the high-grade as compared to low-grade osteosarcoma. We observed no association between vascular density and proliferation index and any clinical parameters. We found an association between HIF-1α expression and metastatic disease and between VEGF expression and female gender.

Original languageEnglish (US)
Pages (from-to)2052-2059
Number of pages8
JournalClinical Orthopaedics and Related Research
Volume466
Issue number9
DOIs
StatePublished - Sep 2008

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Osteosarcoma
Vascular Endothelial Growth Factor A
Cell Proliferation
Factor VIII
Blood Vessels
Hypoxia
Proteins
Transcription Factors
Immunohistochemistry
Staining and Labeling
Neoplasm Metastasis
Phenotype
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Mizobuchi, H., García-Castellano, J. M., Philip, S., Healey, J. H., & Gorlick, R. (2008). Hypoxia markers in human osteosarcoma: An exploratory study. Clinical Orthopaedics and Related Research, 466(9), 2052-2059. https://doi.org/10.1007/s11999-008-0328-y

Hypoxia markers in human osteosarcoma : An exploratory study. / Mizobuchi, Hiroo; García-Castellano, José Manuel; Philip, Shaji; Healey, John H.; Gorlick, Richard.

In: Clinical Orthopaedics and Related Research, Vol. 466, No. 9, 09.2008, p. 2052-2059.

Research output: Contribution to journalArticle

Mizobuchi, H, García-Castellano, JM, Philip, S, Healey, JH & Gorlick, R 2008, 'Hypoxia markers in human osteosarcoma: An exploratory study', Clinical Orthopaedics and Related Research, vol. 466, no. 9, pp. 2052-2059. https://doi.org/10.1007/s11999-008-0328-y
Mizobuchi H, García-Castellano JM, Philip S, Healey JH, Gorlick R. Hypoxia markers in human osteosarcoma: An exploratory study. Clinical Orthopaedics and Related Research. 2008 Sep;466(9):2052-2059. https://doi.org/10.1007/s11999-008-0328-y
Mizobuchi, Hiroo ; García-Castellano, José Manuel ; Philip, Shaji ; Healey, John H. ; Gorlick, Richard. / Hypoxia markers in human osteosarcoma : An exploratory study. In: Clinical Orthopaedics and Related Research. 2008 ; Vol. 466, No. 9. pp. 2052-2059.
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