Hypoxia-inducible factor-1 drives annexin A2 system-mediated perivascular fibrin clearance in oxygen-induced retinopathy in mice

Bihui Huang, Arun B. Deora, Kai Li He, Kang Chen, Guangzhi Sui, Andrew T. Jacovina, Dena Almeida, Peng Hong, Paul Burgman, Katherine A. Hajjar

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Oxygen-induced retinopathy (OIR) is a well-characterized model for retinopathy of prematurity, a disorder that results from rapid microvascular proliferation after exposure of the retina to high oxygen levels. Here, we report that the proliferative phase of OIR requires transcriptional induction of the annexin A2 (A2) gene through the direct action of the hypoxiainducible factor-1 complex. We show, in addition, that A2 stabilizes its binding partner, p11, and promotes OIR-related angiogenesis by enabling clearance of perivascular fibrin. Adenoviral-mediated restoration ofA2 expression restores neovascularization in the oxygen-primed Anxa2-/- retina and reinstates plasmin generation and directed migration in cultured Anxa2 -/- endothelial cells. Systemic depletion of fibrin repairs the neovascular response to high oxygen treatment in the Anxa2-/- retina, whereas inhibition of plasminogen activation dampens angiogenesis under the same conditions. These findings show that the A2 system enables retinal neoangiogenesis in OIR by enhancing perivascular activation of plasmin and remodeling of fibrin. These data suggest new potential approaches to retinal angiogenic disorders on the basis of modulation of perivascular fibrinolysis.

Original languageEnglish (US)
Pages (from-to)2918-2929
Number of pages12
JournalBlood
Volume118
Issue number10
DOIs
StatePublished - Sep 8 2011

Fingerprint

Annexin A2
Hypoxia-Inducible Factor 1
Fibrin
Oxygen
varespladib methyl
Retina
Fibrinolysin
Chemical activation
Retinopathy of Prematurity
Plasminogen
Endothelial cells
Fibrinolysis
Restoration
Repair
Endothelial Cells
Genes
Modulation

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Huang, B., Deora, A. B., He, K. L., Chen, K., Sui, G., Jacovina, A. T., ... Hajjar, K. A. (2011). Hypoxia-inducible factor-1 drives annexin A2 system-mediated perivascular fibrin clearance in oxygen-induced retinopathy in mice. Blood, 118(10), 2918-2929. https://doi.org/10.1182/blood-2011-03-341214

Hypoxia-inducible factor-1 drives annexin A2 system-mediated perivascular fibrin clearance in oxygen-induced retinopathy in mice. / Huang, Bihui; Deora, Arun B.; He, Kai Li; Chen, Kang; Sui, Guangzhi; Jacovina, Andrew T.; Almeida, Dena; Hong, Peng; Burgman, Paul; Hajjar, Katherine A.

In: Blood, Vol. 118, No. 10, 08.09.2011, p. 2918-2929.

Research output: Contribution to journalArticle

Huang, B, Deora, AB, He, KL, Chen, K, Sui, G, Jacovina, AT, Almeida, D, Hong, P, Burgman, P & Hajjar, KA 2011, 'Hypoxia-inducible factor-1 drives annexin A2 system-mediated perivascular fibrin clearance in oxygen-induced retinopathy in mice', Blood, vol. 118, no. 10, pp. 2918-2929. https://doi.org/10.1182/blood-2011-03-341214
Huang, Bihui ; Deora, Arun B. ; He, Kai Li ; Chen, Kang ; Sui, Guangzhi ; Jacovina, Andrew T. ; Almeida, Dena ; Hong, Peng ; Burgman, Paul ; Hajjar, Katherine A. / Hypoxia-inducible factor-1 drives annexin A2 system-mediated perivascular fibrin clearance in oxygen-induced retinopathy in mice. In: Blood. 2011 ; Vol. 118, No. 10. pp. 2918-2929.
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