Hypothermia-Enhanced Human Tumor Cell Radiosensitivity

Steven A. Burton, William R. Paljug, Shalom Kalnicki, E. Day Werts

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Ablation of neoplastic disease by freezing has found increasing utility as a potential therapeutic modality. To assess the effect of cooling temperatures on cellular radiation response, an established human cervical carcinoma cell line (HTB35) was subjected to holding temperatures of 0, 5, or 15°C for up to 24 h before irradiation. Survival was measured by in vitro clonogenic assay of colonies containing at least 50 cells. Cooling for up to 12 h did not significantly decrease survival, but after 24 h survival fell to 75% of control cultures grown at 37°C. X-irradiation immediately after cooling for 24 h resulted in 1.6-fold enhanced radiosensitivity. However, the radiosensitizing effect decayed rapidly if the cooled cells were returned to normal growth temperature for 6 h or longer before irradiation and subculture. Both temperature and cooling duration influenced the radiation response. With 0, 5, or 15°C, radiosensitivity increased after 3, 6, or 12 h, respectively, and progressively rose with up to 24 h of cooling. By flow cytometric analysis, no statistically significant difference was observed in the S-phase fraction between control cells and those cooled to 0°C for 24 h. These data demonstrate cooling-enhanced in vitro radiation sensitivity which is dependent upon cooling temperature, duration, and rewarming interval before irradiation. While cell cycle redistribution does not appear to be a factor in the increased radiosensitivity, differences in the radiation survival curves between cooled versus normothermic cells suggest that diminished capacity for sublethal damage repair may be a significant influence on the changes which were observed.

Original languageEnglish (US)
Pages (from-to)70-78
Number of pages9
JournalCryobiology
Volume35
Issue number1
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

Hypothermia
hypothermia
Radiation Tolerance
Tumors
cooling
Cells
Cooling
Temperature
Survival
irradiation
Radiation
Neoplasms
Irradiation
temperature
Radiation-Sensitizing Agents
Rewarming
cells
S Phase
Freezing
uterine cervical neoplasms

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Burton, S. A., Paljug, W. R., Kalnicki, S., & Werts, E. D. (1997). Hypothermia-Enhanced Human Tumor Cell Radiosensitivity. Cryobiology, 35(1), 70-78.

Hypothermia-Enhanced Human Tumor Cell Radiosensitivity. / Burton, Steven A.; Paljug, William R.; Kalnicki, Shalom; Werts, E. Day.

In: Cryobiology, Vol. 35, No. 1, 08.1997, p. 70-78.

Research output: Contribution to journalArticle

Burton, SA, Paljug, WR, Kalnicki, S & Werts, ED 1997, 'Hypothermia-Enhanced Human Tumor Cell Radiosensitivity', Cryobiology, vol. 35, no. 1, pp. 70-78.
Burton SA, Paljug WR, Kalnicki S, Werts ED. Hypothermia-Enhanced Human Tumor Cell Radiosensitivity. Cryobiology. 1997 Aug;35(1):70-78.
Burton, Steven A. ; Paljug, William R. ; Kalnicki, Shalom ; Werts, E. Day. / Hypothermia-Enhanced Human Tumor Cell Radiosensitivity. In: Cryobiology. 1997 ; Vol. 35, No. 1. pp. 70-78.
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