Hyperthermia-induced apoptosis is independent upon DNA strand breaks in human lymphoid cells

Hwa Jin Jung, Won Hye Ka, Jee Na Hwang, Young Rok Seo

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Heat shock (43°C for 60 minutes) is sufficient to induce apoptosis in a wide number of cell lines. In this study, we asked whether DNA strand breaks are responsible for this phenomenon. Using the highly sensitive comet assay for DNA damage detection, we were unable to demonstrate DNA breaks immediately after heat shock in Raji human lymphoid cells. It showed that DNA breaks were not necessary for hyperthermic apoptosis, since its activity is indicative of DNA lesions. Here, we present a suggestion that a protein(s) is the major target for heat shock apoptosis. We firstly found glycerol, which reportedly stabilizes protein structure, showed a protective effect in Raji cells against hyperthermic apoptosis. In addition, quercetin, which modulates transcription of the heat shock protein family members, enhanced apoptotic death induced by hyperthermia. Furthermore, Raji cells are protected by a pre-mild heat treatment prior to the killing dose of heat shock.

Original languageEnglish (US)
Pages (from-to)345-349
Number of pages5
JournalKorean Journal of Physiology and Pharmacology
Volume8
Issue number6
StatePublished - Dec 1 2004

Keywords

  • Apoptosis
  • Comet assay
  • DNA damage
  • Heat shock protein
  • Hyperthermia

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Fingerprint Dive into the research topics of 'Hyperthermia-induced apoptosis is independent upon DNA strand breaks in human lymphoid cells'. Together they form a unique fingerprint.

  • Cite this