Hyperpolarized [5-13C,4,4-2H2,5-15N]-L-glutamine provides a means of annotating in vivo metabolic utilization of glutamine

Roozbeh Eskandari, Nathaniel Kim, Arsen Mamakhanyan, Michelle Saoi, Guannan Zhang, Marjan Berisaj, Kristin L. Granlund, Alex J. Poot, Justin Cross, Craig B. Thompson, Kayvan R. Keshari

Research output: Contribution to journalArticlepeer-review

Abstract

SignificanceGlutamine is the most abundant amino acid in human plasma, although it is challenging to determine glutamine's metabolic fate noninvasively. In this work, we utilize established chemical methods to develop a platform for imaging glutamine metabolism using hyperpolarized magnetic resonance imaging. Using this strategy, we are able to spatially measure glutaminolysis in vivo as well as develop a biomarker for the inhibition of glutaminase. Combining this biomarker with isotope tracing metabolomics connects this inhibition to reduced glutamine contribution to the tricarboxylic acid cycle. This provides an approach for future imaging of glutamine metabolism in humans.

Original languageEnglish (US)
Pages (from-to)e2120595119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number19
DOIs
StatePublished - May 10 2022

Keywords

  • cancer metabolism
  • magnetic resonance imaging
  • metabolic imaging
  • pancreatic cancer

ASJC Scopus subject areas

  • General

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