Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry

Janna G. Kiselar, Steven C. Almo, Paul A. Janmey, Mark R. Chance

Research output: Contribution to conferencePaper

Abstract

The Ca 2+ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca 2+ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.

Original languageEnglish (US)
Pages363-364
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Kiselar, J. G., Almo, S. C., Janmey, P. A., & Chance, M. R. (2002). Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry. 363-364. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.