Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry

Janna G. Kiselar, Steven C. Almo, Paul A. Janmey, Mark R. Chance

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The Ca 2+ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca 2+ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages363-364
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Gelsolin
Radiolysis
Synchrotrons
Hydroxyl Radical
Mass spectrometry
X rays
Proteolysis
Synchrotron radiation
Sulfur
Amino Acids
Oxidation
Peptides
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Kiselar, J. G., Almo, S. C., Janmey, P. A., & Chance, M. R. (2002). Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 363-364)

Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry. / Kiselar, Janna G.; Almo, Steven C.; Janmey, Paul A.; Chance, Mark R.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 363-364.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kiselar, JG, Almo, SC, Janmey, PA & Chance, MR 2002, Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 363-364, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Kiselar JG, Almo SC, Janmey PA, Chance MR. Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 363-364
Kiselar, Janna G. ; Almo, Steven C. ; Janmey, Paul A. ; Chance, Mark R. / Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 363-364
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