Abstract
The Ca 2+ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca 2+ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.
Original language | English (US) |
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Pages | 363-364 |
Number of pages | 2 |
State | Published - 2002 |
Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
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Country/Territory | United States |
City | Orlando, FL |
Period | 6/2/02 → 6/6/02 |
ASJC Scopus subject areas
- Spectroscopy