Hydrogen-deuterium exchange kinetics of the amide protons of oxytocin studied by nuclear magnetic resonance

Eric M. Krauss, David Cowburn

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The contribution of intramolecular hydrogen bonding to the solution structure of oxytocin was evaluated by study of amide hydrogen exchange rates in D2O by Fourier transform 1H NMR spectroscopy. Resolution enhancement filtering was employed in the determination of individual pseudo-first-order rate constants. Apparent barriers to exchange of 0.5 and 0.6 kcal mol-1 were measured for Asn5 and Cys6 peptide NH, respectively. The slowing is best explained by steric hindrance to solvent access in the case of Asn5, while for the Cys6 participation in a weak intramolecular hydrogen bond is possible. Fourfold acceleration of base-catalyzed exchange was observed for Tyr2 NH; it is proposed that this is the result of electronic effects induced by hydrogen bonding of Cys1 C=O, either to Cys6 NH or to the N-terminal amino group. Exchange proceeds near the random coil limit for each of the remaining residues. Comparison with exchange data for the model tripeptide N-acetyl-L-prolyl-L-leucylglycinamide demonstrates no evidence of noncovalent association of the tocin ring with the tripeptide tail of the hormone.

Original languageEnglish (US)
Pages (from-to)671-679
Number of pages9
JournalBiochemistry
Volume20
Issue number4
StatePublished - 1981
Externally publishedYes

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Deuterium
Oxytocin
Hydrogen Bonding
Amides
Protons
Hydrogen
Hydrogen bonds
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Kinetics
Fourier Analysis
Electronic data interchange
Hormones
Peptides
Nuclear magnetic resonance spectroscopy
Rate constants
Fourier transforms
Association reactions
Proton Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hydrogen-deuterium exchange kinetics of the amide protons of oxytocin studied by nuclear magnetic resonance. / Krauss, Eric M.; Cowburn, David.

In: Biochemistry, Vol. 20, No. 4, 1981, p. 671-679.

Research output: Contribution to journalArticle

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N2 - The contribution of intramolecular hydrogen bonding to the solution structure of oxytocin was evaluated by study of amide hydrogen exchange rates in D2O by Fourier transform 1H NMR spectroscopy. Resolution enhancement filtering was employed in the determination of individual pseudo-first-order rate constants. Apparent barriers to exchange of 0.5 and 0.6 kcal mol-1 were measured for Asn5 and Cys6 peptide NH, respectively. The slowing is best explained by steric hindrance to solvent access in the case of Asn5, while for the Cys6 participation in a weak intramolecular hydrogen bond is possible. Fourfold acceleration of base-catalyzed exchange was observed for Tyr2 NH; it is proposed that this is the result of electronic effects induced by hydrogen bonding of Cys1 C=O, either to Cys6 NH or to the N-terminal amino group. Exchange proceeds near the random coil limit for each of the remaining residues. Comparison with exchange data for the model tripeptide N-acetyl-L-prolyl-L-leucylglycinamide demonstrates no evidence of noncovalent association of the tocin ring with the tripeptide tail of the hormone.

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