Hydrogen-bonding conformations of tyrosine B10 tailor the hemeprotein reactivity of ferryl species

Walleska De Jesús-Bonilla, Anthony Cruz, Ariel Lewis, José Cerda, Daniel E. Bacelo, Carmen L. Cadilla, Juan López-Garriga

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8 Scopus citations

Abstract

Ferryl compounds [Fe(IV) = O] in living organisms play an essential role in the radical catalytic cycle and degradation processes of hemeproteins. We studied the reactions between H2O2 and hemoglobin II (HbII) (GlnE7, TyrB10, PheCD1, PheE11), recombinant hemoglobin I (HbI) (GlnE7, PheB10, PheCD1, PheE11), and the HbI PheB10Tyr mutant of L. pectinata. We found that the tyrosine residue in the B10 position tailors, in two very distinct ways, the reactivity of the ferryl species, compounds I and II. First, increasing the reaction pH from 4.86 to 7.50, and then to 11.2, caused the the second-order rate constant for HbII to decrease from 141.60 to 77.78 M -1 s-1, and to 2.96 M-1 s-1, respectively. This pH dependence is associated with the disruption of the heme-tyrosine (603 nm) protein moiety, which controls the access of the H 2O2 to the hemeprotein active center, thus regulating the formation of the ferryl species. Second, the presence of compound I was evident in the UV-vis spectra (648-nm band) in the reactions of HbI and recombinant HbI with H2O2, This band, however, is completely absent in the analogous reaction with HbII and the HbI PheB10Tyr mutant. Therefore, the existence of a hydrogen-bonding network between the heme pocket amino acids (i.e., TyrB10) and the ferryl compound I created a path much faster than 3.0×10-2 s-1 for the decay of compound I to compound II. Furthermore, the decay of the heme ferryl compound I to compound II was independent of the proximal HisF8 trans-ligand strength. Thus, the pH dependence of the heme-tyrosine moiety complex determined the overall reaction rate of the oxidative reaction limiting the interaction with H2O2 at neutral pH. The hydrogen-bonding strength between the TyrB10 and the heme ferryl species suggests the presence of a cycle where the ferryl consumption by the ferric heme increases significantly the pseudoperoxidase activity of these hemeproteins.

Original languageEnglish (US)
Pages (from-to)334-342
Number of pages9
JournalJournal of Biological Inorganic Chemistry
Volume11
Issue number3
DOIs
StatePublished - Apr 1 2006

Keywords

  • Ferryl species
  • Hydrogen peroxide
  • Lucina pectinata hemoglobins
  • Proximal HisF8 trans effect
  • Tyrosine B10

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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    De Jesús-Bonilla, W., Cruz, A., Lewis, A., Cerda, J., Bacelo, D. E., Cadilla, C. L., & López-Garriga, J. (2006). Hydrogen-bonding conformations of tyrosine B10 tailor the hemeprotein reactivity of ferryl species. Journal of Biological Inorganic Chemistry, 11(3), 334-342. https://doi.org/10.1007/s00775-006-0082-0