Hydration of Cellobial by Exo and Endo-Type Cellulases: Evidence for Catalytic Flexibility of Glycosylases

Takahisa Kanda, Curtis F. Brewer, Gentaro Okada, Edward J. Hehre

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

New insight has been obtained into the catalytic capabilities of cellulase. Essentially homogeneous preparations of exo (or Avicelase-) type and endo (or CMCase-) type cellulases from Irpex lacteus and Aspergillus niger, respectively, were shown to hydrate the enolic bond of cellobial to form 2-deoxycellobiose. The A. niger enzyme also synthesized a small amount of a 2-deoxycellobiosyl-transfer product from cellobial. By use of digests conducted in deuterated buffer and 1H NMR spectra for product analysis, both cellulases were found to protonate (deuterate) the double bond of cellobial from below the si face of the D-glucal moiety, i.e., from a direction opposite that assumed for protonation of the β-D-glycosidic linkages of cellulose and cellodextrins. The exo enzyme, which hydrolyzes the latter substrates primarily to cellobiose, rapidly catalyzed cellobial hydration to produce the β-anomer of β-D-glucopyranosyl(l→4)-2-deoxy-D-glucose-2(e)-d. The A. niger cellulase produced the same 2-deoxycellobiose-d from cellobial, though too slowly for its configuration to be determined. However, evidence was obtained for the formation of a β-2-deoxycellobiosyl-d-D-glucose-transfer product by the enzyme. Thus, it is likely that all of the observed reactions with cellobial represent trans additions at the double bond. In any case, the anomeric configuration of products is created de novo. Separate mechanisms are described for the reaction of cellobial hydration and for the stereochemically different reaction of cellulose hydrolysis catalyzed by the present enzymes, assuming an arrangement of their catalytic groups analogous to that found in lysozyme. The results with the cellulases add to growing evidence for the view that glycosylases have catalytic groups that are functionally flexible beyond the needs of the principle of microscopic reversibility and, hence, the potential to act upon different substrates by different mechanisms.

Original languageEnglish (US)
Pages (from-to)1159-1165
Number of pages7
JournalBiochemistry
Volume25
Issue number5
DOIs
StatePublished - Mar 1986

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Hydration of Cellobial by Exo and Endo-Type Cellulases: Evidence for Catalytic Flexibility of Glycosylases'. Together they form a unique fingerprint.

  • Cite this