Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding

Tracy L. Rankin, Zhi B. Tong, Philip E. Castle, Eric Lee, Robert Gore-Langton, Lawrence M. Nelson, Jurrien Dean

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The mammalian zona pellucida surrounding ovulated eggs mediates sperm binding at fertilization, provides a postfertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct. Although the three zona proteins (ZP1, ZP2, ZP3) are well conserved, mammalian fertilization is relatively specific and human sperm do not bind to the mouse zona pellucida. There are considerable in vitro data that ZP3 acts as a primary sperm adhesion molecule in mice and, by analogy, a similar role has been postulated for human ZP3. Genetically altered mice lacking ZP3 (Zp3(tm/tm)) do not form a zona pellucida and are infertile. To rescue this phenotype, transgenic mice expressing human ZP3 (67% identical to mouse ZP3) were produced and bred with Zp3(tm/tm) null mice. The resultant human ZP3 rescue females had chimeric zonae pellucidae composed of mouse ZP1, mouse ZP2 and human ZP3. Human ZP3 expressed in mouse oocytes had an apparent mass (64 kDa) indistinguishable from native human ZP3 and distinct from mouse ZP3 (83 kDa). Despite the presence of human ZP3, human sperm did not bind to the chimeric zona pellucida, and notwithstanding the absence of mouse ZP3, mouse sperm bound to ovulated eggs in vitro and fertility was restored in vivo. These data have implications regarding the molecular basis of mouse and human sperm binding to their respective zonae pellucidae.

Original languageEnglish (US)
Pages (from-to)2415-2424
Number of pages10
JournalDevelopment
Volume125
Issue number13
StatePublished - Jul 1998
Externally publishedYes

Fingerprint

Fertility
Spermatozoa
Zona Pellucida
Fertilization
Eggs
Oviducts
Herpes Zoster
Population Groups
Transgenic Mice
Oocytes
Phenotype

Keywords

  • Human fertilization
  • Mouse
  • Mouse fertility
  • Order-specific sperm binding
  • Sperm-egg interaction
  • Transgene
  • Zona pellucida

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Rankin, T. L., Tong, Z. B., Castle, P. E., Lee, E., Gore-Langton, R., Nelson, L. M., & Dean, J. (1998). Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development, 125(13), 2415-2424.

Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. / Rankin, Tracy L.; Tong, Zhi B.; Castle, Philip E.; Lee, Eric; Gore-Langton, Robert; Nelson, Lawrence M.; Dean, Jurrien.

In: Development, Vol. 125, No. 13, 07.1998, p. 2415-2424.

Research output: Contribution to journalArticle

Rankin, TL, Tong, ZB, Castle, PE, Lee, E, Gore-Langton, R, Nelson, LM & Dean, J 1998, 'Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding', Development, vol. 125, no. 13, pp. 2415-2424.
Rankin TL, Tong ZB, Castle PE, Lee E, Gore-Langton R, Nelson LM et al. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development. 1998 Jul;125(13):2415-2424.
Rankin, Tracy L. ; Tong, Zhi B. ; Castle, Philip E. ; Lee, Eric ; Gore-Langton, Robert ; Nelson, Lawrence M. ; Dean, Jurrien. / Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. In: Development. 1998 ; Vol. 125, No. 13. pp. 2415-2424.
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