Human vascular smooth muscle cells possess functional CCR5

Alison D. Schecter, Tina M. Calderon, Adriane B. Berman, Carrie M. McManus, John T. Fallon, Maria Rossikhina, Weixin Zhao, George Christ, Joan W. Berman, Mark B. Taubman

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

CC chemokine receptors are important modulators of inflammation. Although CC chemokine receptors have been found predominantly on leukocytes, recent studies have suggested that vascular smooth muscle cells respond to CC chemokines. We now report that human smooth muscle cells express CCR5, a co- receptor for human immunodeficiency virus. CCR5 mRNA was detectable by RNA blot hybridization in human aortic and coronary artery smooth muscle cells. The cDNA generated by reverse transcription-polymerase chain reaction from aortic smooth muscle cells had 100% identity throughout the entire coding region with the CCR5 cloned from THP-1 cells. By immunohistochemistry, CCR5 and the CCR5 ligand, macrophage inflammatory protein-1β (MIP-1β), were detected in smooth muscle cells and macrophages of the atherosclerotic plaque. In smooth muscle cell culture, MIP-1β induced a significant increase in intracellular calcium concentrations, which was blocked by an antibody to CCR5. In addition, MIP-1β caused a calcium-dependent increase in tissue factor activity. Tissue factor is the initiator of coagulation and is thought to play a key role in arterial thrombosis. These data suggest that human arterial smooth muscle cells express functional CCR5 receptors and MIP-1β is an agonist for these cells.

Original languageEnglish (US)
Pages (from-to)5466-5471
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number8
DOIs
StatePublished - Feb 25 2000

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Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Macrophage Inflammatory Proteins
Cells
CCR Receptors
Thromboplastin
CCR5 Receptors
Calcium
CC Chemokines
Macrophages
Polymerase chain reaction
Atherosclerotic Plaques
Transcription
Coagulation
Viruses
Cell culture
Modulators
Reverse Transcription
Coronary Vessels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Schecter, A. D., Calderon, T. M., Berman, A. B., McManus, C. M., Fallon, J. T., Rossikhina, M., ... Taubman, M. B. (2000). Human vascular smooth muscle cells possess functional CCR5. Journal of Biological Chemistry, 275(8), 5466-5471. https://doi.org/10.1074/jbc.275.8.5466

Human vascular smooth muscle cells possess functional CCR5. / Schecter, Alison D.; Calderon, Tina M.; Berman, Adriane B.; McManus, Carrie M.; Fallon, John T.; Rossikhina, Maria; Zhao, Weixin; Christ, George; Berman, Joan W.; Taubman, Mark B.

In: Journal of Biological Chemistry, Vol. 275, No. 8, 25.02.2000, p. 5466-5471.

Research output: Contribution to journalArticle

Schecter, AD, Calderon, TM, Berman, AB, McManus, CM, Fallon, JT, Rossikhina, M, Zhao, W, Christ, G, Berman, JW & Taubman, MB 2000, 'Human vascular smooth muscle cells possess functional CCR5', Journal of Biological Chemistry, vol. 275, no. 8, pp. 5466-5471. https://doi.org/10.1074/jbc.275.8.5466
Schecter AD, Calderon TM, Berman AB, McManus CM, Fallon JT, Rossikhina M et al. Human vascular smooth muscle cells possess functional CCR5. Journal of Biological Chemistry. 2000 Feb 25;275(8):5466-5471. https://doi.org/10.1074/jbc.275.8.5466
Schecter, Alison D. ; Calderon, Tina M. ; Berman, Adriane B. ; McManus, Carrie M. ; Fallon, John T. ; Rossikhina, Maria ; Zhao, Weixin ; Christ, George ; Berman, Joan W. ; Taubman, Mark B. / Human vascular smooth muscle cells possess functional CCR5. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 8. pp. 5466-5471.
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