Human umbilical cord mesenchymal stem cells inhibit glioma growth

Shuo Liang, Hong Liang Jiao, Lian Kai Chi, Xin Yi Shi, A. Ming Liang, Yi Tian, Jiao Ling Han, Shan Shan Ma, Bo Yang, Fangxia Guan

Research output: Contribution to journalArticle

Abstract

Background: Wnt signaling pathway is a key to occurrence and development of tumor. Several studies have demonstrated that Dickkopf-1 can block intracellular Wnt signalling transmission. Objective: To investigate the inhibitory effect and underlying mechanism of human umbilical cord mesenchymal stem cells on C6 glioma growth by a co-culture system in vitro. Methods: C6 glioma cells were cultured with human umbilical cord mesenchymal stem cells conditioned medium under different concentrations. Cell Counting Kit-8 and flow cytometry analysis were performed to investigate cell proliferation and cell cycle status. Western blotting was used to detect the expression of β-catenin and c-Myc in C6 cells treated with human umbilical cord mesenchymal stem cells conditioned medium. Enzyme-linked immunosorbent assay was adopted to examine the level of Dickkopf-1 secreted by human umbilical cord mesenchymal stem cells. Dickkopf-1 in human umbilical cord mesenchymal stem cells conditioned medium was neutralized by anti-Dickkopf-1 antibody. The effects of antibody neutralization were also determined by the above methods. Results and Conclusion: Human umbilical cord mesenchymal stem cells could inhibit C6 cell expansion and arrest the cell cycle in G0-G1 phase. The expression levels of β-catenin and c-Myc were down-regulated in C6 cells after treatment with human umbilical cord mesenchymal stem cells conditioned medium. The levels of secreted protein Dickkopf-1 were positively correlated with concentrations of human umbilical cord mesenchymal stem cells conditioned medium. The inhibitory effect of human umbilical cord mesenchymal stem cells on C6 cell proliferation was enhanced as the concentration of Dickkopf-1 in human umbilical cord mesenchymal stem cells conditioned medium increased. When Dickkopf-1 was neutralized by anti-Dickkopf-1 antibody, the suppressing effect was attenuated. It demonstrated that human umbilical cord mesenchymal stem cells could inhibit glioma cell growth via secreting the soluble factors, such as Dickkopf-1.

Original languageEnglish (US)
Pages (from-to)9179-9185
Number of pages7
JournalChinese Journal of Tissue Engineering Research
Volume16
Issue number49
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

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Umbilical Cord
Stem cells
Mesenchymal Stromal Cells
Glioma
Conditioned Culture Medium
Growth
Antibodies
Catenins
Cell proliferation
Cells
Cell Proliferation
Cell Cycle Resting Phase
Immunosorbents
Wnt Signaling Pathway
Flow cytometry
G1 Phase
Cell growth
Coculture Techniques
Cell Cycle Checkpoints
Tumors

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biomedical Engineering
  • Orthopedics and Sports Medicine

Cite this

Human umbilical cord mesenchymal stem cells inhibit glioma growth. / Liang, Shuo; Jiao, Hong Liang; Chi, Lian Kai; Shi, Xin Yi; Liang, A. Ming; Tian, Yi; Han, Jiao Ling; Ma, Shan Shan; Yang, Bo; Guan, Fangxia.

In: Chinese Journal of Tissue Engineering Research, Vol. 16, No. 49, 01.12.2013, p. 9179-9185.

Research output: Contribution to journalArticle

Liang, S, Jiao, HL, Chi, LK, Shi, XY, Liang, AM, Tian, Y, Han, JL, Ma, SS, Yang, B & Guan, F 2013, 'Human umbilical cord mesenchymal stem cells inhibit glioma growth', Chinese Journal of Tissue Engineering Research, vol. 16, no. 49, pp. 9179-9185. https://doi.org/10.3969/j.issn.2095-4344.2012.49.011
Liang, Shuo ; Jiao, Hong Liang ; Chi, Lian Kai ; Shi, Xin Yi ; Liang, A. Ming ; Tian, Yi ; Han, Jiao Ling ; Ma, Shan Shan ; Yang, Bo ; Guan, Fangxia. / Human umbilical cord mesenchymal stem cells inhibit glioma growth. In: Chinese Journal of Tissue Engineering Research. 2013 ; Vol. 16, No. 49. pp. 9179-9185.
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abstract = "Background: Wnt signaling pathway is a key to occurrence and development of tumor. Several studies have demonstrated that Dickkopf-1 can block intracellular Wnt signalling transmission. Objective: To investigate the inhibitory effect and underlying mechanism of human umbilical cord mesenchymal stem cells on C6 glioma growth by a co-culture system in vitro. Methods: C6 glioma cells were cultured with human umbilical cord mesenchymal stem cells conditioned medium under different concentrations. Cell Counting Kit-8 and flow cytometry analysis were performed to investigate cell proliferation and cell cycle status. Western blotting was used to detect the expression of β-catenin and c-Myc in C6 cells treated with human umbilical cord mesenchymal stem cells conditioned medium. Enzyme-linked immunosorbent assay was adopted to examine the level of Dickkopf-1 secreted by human umbilical cord mesenchymal stem cells. Dickkopf-1 in human umbilical cord mesenchymal stem cells conditioned medium was neutralized by anti-Dickkopf-1 antibody. The effects of antibody neutralization were also determined by the above methods. Results and Conclusion: Human umbilical cord mesenchymal stem cells could inhibit C6 cell expansion and arrest the cell cycle in G0-G1 phase. The expression levels of β-catenin and c-Myc were down-regulated in C6 cells after treatment with human umbilical cord mesenchymal stem cells conditioned medium. The levels of secreted protein Dickkopf-1 were positively correlated with concentrations of human umbilical cord mesenchymal stem cells conditioned medium. The inhibitory effect of human umbilical cord mesenchymal stem cells on C6 cell proliferation was enhanced as the concentration of Dickkopf-1 in human umbilical cord mesenchymal stem cells conditioned medium increased. When Dickkopf-1 was neutralized by anti-Dickkopf-1 antibody, the suppressing effect was attenuated. It demonstrated that human umbilical cord mesenchymal stem cells could inhibit glioma cell growth via secreting the soluble factors, such as Dickkopf-1.",
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AU - Liang, Shuo

AU - Jiao, Hong Liang

AU - Chi, Lian Kai

AU - Shi, Xin Yi

AU - Liang, A. Ming

AU - Tian, Yi

AU - Han, Jiao Ling

AU - Ma, Shan Shan

AU - Yang, Bo

AU - Guan, Fangxia

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N2 - Background: Wnt signaling pathway is a key to occurrence and development of tumor. Several studies have demonstrated that Dickkopf-1 can block intracellular Wnt signalling transmission. Objective: To investigate the inhibitory effect and underlying mechanism of human umbilical cord mesenchymal stem cells on C6 glioma growth by a co-culture system in vitro. Methods: C6 glioma cells were cultured with human umbilical cord mesenchymal stem cells conditioned medium under different concentrations. Cell Counting Kit-8 and flow cytometry analysis were performed to investigate cell proliferation and cell cycle status. Western blotting was used to detect the expression of β-catenin and c-Myc in C6 cells treated with human umbilical cord mesenchymal stem cells conditioned medium. Enzyme-linked immunosorbent assay was adopted to examine the level of Dickkopf-1 secreted by human umbilical cord mesenchymal stem cells. Dickkopf-1 in human umbilical cord mesenchymal stem cells conditioned medium was neutralized by anti-Dickkopf-1 antibody. The effects of antibody neutralization were also determined by the above methods. Results and Conclusion: Human umbilical cord mesenchymal stem cells could inhibit C6 cell expansion and arrest the cell cycle in G0-G1 phase. The expression levels of β-catenin and c-Myc were down-regulated in C6 cells after treatment with human umbilical cord mesenchymal stem cells conditioned medium. The levels of secreted protein Dickkopf-1 were positively correlated with concentrations of human umbilical cord mesenchymal stem cells conditioned medium. The inhibitory effect of human umbilical cord mesenchymal stem cells on C6 cell proliferation was enhanced as the concentration of Dickkopf-1 in human umbilical cord mesenchymal stem cells conditioned medium increased. When Dickkopf-1 was neutralized by anti-Dickkopf-1 antibody, the suppressing effect was attenuated. It demonstrated that human umbilical cord mesenchymal stem cells could inhibit glioma cell growth via secreting the soluble factors, such as Dickkopf-1.

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