Human umbilical cord mesenchymal stem cells inhibit C6 glioma growth via secretion of dickkopf-1 (DKK1)

Shanshan Ma, Shuo Liang, Hongliang Jiao, Liankai Chi, Xinyi Shi, Yi Tian, Bo Yang, Fangxia Guan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) represent a potential therapeutic target for glioma. We determined the molecular mechanism of inhibitory effect of human umbilical cord-derived MSCs (hUC-MSCs) on the growth of C6 glioma cells. We demonstrated that hUC-MSCs inhibited C6 cell growth and modulated the cell cycle to G0/G1 phase. The expression of β-catenin and c-Myc was downregulated in C6 cells by conditioned media from hUC-MSCs, and the levels of secreted DKK1 were positively correlated with concentrations of hUCMSCs-CM. The inhibitory effect of hUC-MSCs on C6 cell proliferation was enhanced as the concentration of DKK1 in hUCMSCs-CM increased. When DKK1 was neutralized by anti-DKK1 antibody, the inhibitory effect of hUC-MSCs on C6 cells was attenuated. Furthermore, we found that conditioned media from hUC-MSCs transfection with siRNA targeting DKK1 mRNA or pEGFPN1-DKK1 plasmid lost or enhanced the abilities to regulate the Wnt signaling in C6 cells. Therefore, hUC-MSCs inhibited C6 glioma cell growth via secreting DKK1, an inhibitor of Wnt pathway, may represent a novel therapeutic strategy for malignant glioma.

Original languageEnglish (US)
Pages (from-to)277-286
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume385
Issue number1-2
DOIs
StatePublished - Jan 2014
Externally publishedYes

Fingerprint

Umbilical Cord
Stem cells
Mesenchymal Stromal Cells
Glioma
Growth
Cell growth
Conditioned Culture Medium
Catenins
Cell Cycle Resting Phase
Wnt Signaling Pathway
Cell proliferation
G1 Phase
Small Interfering RNA
Transfection
Cell Cycle
Plasmids
Down-Regulation
Cells
Cell Proliferation
Messenger RNA

Keywords

  • DKK1
  • Glioma
  • Human umbilical cord
  • Inhibition
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Human umbilical cord mesenchymal stem cells inhibit C6 glioma growth via secretion of dickkopf-1 (DKK1). / Ma, Shanshan; Liang, Shuo; Jiao, Hongliang; Chi, Liankai; Shi, Xinyi; Tian, Yi; Yang, Bo; Guan, Fangxia.

In: Molecular and Cellular Biochemistry, Vol. 385, No. 1-2, 01.2014, p. 277-286.

Research output: Contribution to journalArticle

Ma, Shanshan ; Liang, Shuo ; Jiao, Hongliang ; Chi, Liankai ; Shi, Xinyi ; Tian, Yi ; Yang, Bo ; Guan, Fangxia. / Human umbilical cord mesenchymal stem cells inhibit C6 glioma growth via secretion of dickkopf-1 (DKK1). In: Molecular and Cellular Biochemistry. 2014 ; Vol. 385, No. 1-2. pp. 277-286.
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