Human testicular glutathione S-transferases: Insights into tissue-specific expression of the diverse subunit classes

Irving Listowsky, Jonathan D. Rowe, Yury V. Patskovsky, Tatyana L. Tchaikovskaya, Naoaki Shintani, Elena Novikova, Edward Nieves

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cytosolic glutathione S-transferase (GST) subunits from human testis were resolved by HPLC and unambiguously identified by combined use of peptide sequence-specific antisera and electrospray ionization mass spectrometry (ESI MS). Allelic variants of hGSTP1, hGSTM1 and hGSTA2 were distinguished on the basis of observed differences in their molecular masses. Relative amounts of the multiple different subunit types in various human tissues were determined from HPLC profiles. From this type of analysis, tissues from hGSTM1 null allele individuals were readily discerned at the protein level; liver was the only tissue in which the hGSTM1 subunit was the major μ-class GST. hGSTM4 and hGSTM5 subunits were found at very low levels in all tissues examined. By far the tissue richest in the unique hGSTM3 subunit was testis, although brain also has significant levels. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalChemico-Biological Interactions
Volume111-112
DOIs
StatePublished - Apr 24 1998

Fingerprint

Glutathione Transferase
Tissue
Testis
High Pressure Liquid Chromatography
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Molecular mass
Liver
Mass spectrometry
Immune Sera
Brain
Alleles
Peptides
Proteins

Keywords

  • Glutathione transferase
  • HPLC
  • Mass spectrometry
  • Testis

ASJC Scopus subject areas

  • Toxicology

Cite this

Human testicular glutathione S-transferases : Insights into tissue-specific expression of the diverse subunit classes. / Listowsky, Irving; Rowe, Jonathan D.; Patskovsky, Yury V.; Tchaikovskaya, Tatyana L.; Shintani, Naoaki; Novikova, Elena; Nieves, Edward.

In: Chemico-Biological Interactions, Vol. 111-112, 24.04.1998, p. 103-112.

Research output: Contribution to journalArticle

Listowsky, Irving ; Rowe, Jonathan D. ; Patskovsky, Yury V. ; Tchaikovskaya, Tatyana L. ; Shintani, Naoaki ; Novikova, Elena ; Nieves, Edward. / Human testicular glutathione S-transferases : Insights into tissue-specific expression of the diverse subunit classes. In: Chemico-Biological Interactions. 1998 ; Vol. 111-112. pp. 103-112.
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