Human sperm do not bind to rat zonae pellucidae despite the presence of four homologous glycoproteins

Tanya Hoodbhoy, Saurabh Joshi, Emily S. Boja, Suzannah A. Williams, Pamela Stanley, Jurrien Dean

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The specificity of sperm-egg recognition in mammals is mediated primarily by the zona pellucida surrounding ovulated eggs. Mouse sperm are quite promiscuous and bind to human eggs, but human spermatozoa will not bind to mouse eggs. The mouse zona pellucida contains three glycoproteins, ZP1, ZP2, and ZP3, which are conserved in rat and human. The recent observation that human zonae pellucidae contain a fourth protein raises the possibility that the presence of four zona proteins will support human sperm binding. Using mass spectrometry, four proteins that are similar in size and share 62-70% amino acid identity with human ZP1, ZP2, ZP3, and ZP4/ZPB were detected in rat zonae pellucidae. However, although mouse and rat spermatozoa bind to eggs from each rodent, human sperm bind to neither, and the presence of human follicular fluid did not alter the specificity of sperm binding. In addition, mutant mouse eggs lacking hybrid/complex N-glycans or deficient in Core 2 O-glycans were no more able to support human sperm binding than normal mouse eggs. These data suggest that the presence of four zona proteins are not sufficient to support human sperm binding to rodent eggs and that additional determinants must be responsible for taxon-specific fertilization among mammals.

Original languageEnglish (US)
Pages (from-to)12721-12731
Number of pages11
JournalJournal of Biological Chemistry
Volume280
Issue number13
DOIs
StatePublished - Apr 1 2005

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Zona Pellucida
Spermatozoa
Rats
Glycoproteins
Eggs
Mammals
Polysaccharides
Proteins
Mass spectrometry
Herpes Zoster
Rodentia
Amino Acids
Fluids
Follicular Fluid
Fertilization
Ovum
Mass Spectrometry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Human sperm do not bind to rat zonae pellucidae despite the presence of four homologous glycoproteins. / Hoodbhoy, Tanya; Joshi, Saurabh; Boja, Emily S.; Williams, Suzannah A.; Stanley, Pamela; Dean, Jurrien.

In: Journal of Biological Chemistry, Vol. 280, No. 13, 01.04.2005, p. 12721-12731.

Research output: Contribution to journalArticle

Hoodbhoy, Tanya ; Joshi, Saurabh ; Boja, Emily S. ; Williams, Suzannah A. ; Stanley, Pamela ; Dean, Jurrien. / Human sperm do not bind to rat zonae pellucidae despite the presence of four homologous glycoproteins. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 13. pp. 12721-12731.
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