Human serum albumin microspheres approximate initial organspecific biodistributions of transplanted hepatocytes and are effective cell surrogates for safety studies

Pankaj Rajvanshi, Kuldeep K. Bhargava, Menes Afriyie, Maria V. Camaya, S. Gagandeep, Srinivasa Rao G. Vasa, Christopher J. Palestro, Sanjeev Gupta

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Liver repopulation with transplanted hepatocytes will generate novel cell-based therapies, although translocation of transplanted cells into lungs through portasystemic shunts has the potential for embolic complications. To facilitate safety analysis of hepatocyte transplantation, we wished to obtain effective cell surrogates and analyzed biodistributions of similarly sized 99mTc-labeled human serum albumin microspheres and rat hepatocytes. Image analysis with dual 99mTc and 111In labels indicated that cells and microspheres were similarly distributed in the liver when injected into normal rats via the spleen. Also, their distributions were similar when injected via a femoral vein or the superior mesenteric vein with cells and microspheres localizing in lungs or liver, respectively. Upon intraportal injection in rats with portal hypertension, microspheres localized in both liver and lungs, consistent with portasystemic shunting. These data demonstrate that human serum albumin microspheres are effective cell surrogates for approximating the safety of hepatocyte transplantation and should be clinically useful.

Original languageEnglish (US)
Pages (from-to)275-283
Number of pages9
JournalCell Transplantation
Volume7
Issue number3
DOIs
Publication statusPublished - May 1 1998

    Fingerprint

Keywords

  • Hepatocyte transplantation
  • Human serum albumin
  • Liver
  • Microspheres
  • Portasystemic shunting
  • Pulmonary embolism

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this