Human parathyroid autografts

Comparison of function in vivo and vitro

E. M. Brown, M. F. Brennan, A. R. Broadus, S. J. Marx, D. G. Gardner, Allen M. Spiegel, R. W. Downs, M. F. Attie, G. D. Aurbach

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Suppressibility of parathyroid function by calcium was compared in vivo and in vitro for five patients with parathyroid autografts to the forearm. Suppressibility of parathyroid secretion was studied in vitro with dispersed parathyroid cells and in vivo by determining nephrogenous cAMP (NcAMP) excretion and parathyroid hormone (PTH) concentration in the antecubital vein. In four patients with primary parathyroid hyperplasia, a graded calcium infusion caused progressive inhibition of NcAMP and PTH release from the autograft. Maximal inhibition of 90 to >95%, respectively, was achieved with total calcium concentrations that reached maxima of 2.65-3.1 mM. PTH release from dispersed parathyroid cells of these patients was suppressed 70-80% by ionized calcium concentrations of 1.5-2.0 mM. A single patient with a transplanted adenoma showed a 17-39% suppression of NcAMP and a 20-60% inhibition of antecubital venous PTH in two separate calcium infusions, as well as a 20-30% inhibition by calcium of PTH release from dispersed parathyroid cells in vitro. The present results indicate a close correlation between PTH in the venous effluent from parathyroid autografts and NcAMP. Suppression of parathyroid function by elevated calcium concentration was very similar in vivo and in vitro, suggesting further that dispersed human parathyroid cells provide a valid representation of parathyroid suppressibility in vivo. Taken in conjunction with our previous studies with dispersed parathyroid cells in vitro and in patients given calcium infusions before parathyroidectomy, these studies are consistent with distinct calcium sensitivity in parathyroid adenoma vs. primary hyperplasia.

Original languageEnglish (US)
Pages (from-to)648-654
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume48
Issue number4
StatePublished - 1979
Externally publishedYes

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Autografts
Calcium
Parathyroid Hormone
Hyperplasia
In Vitro Techniques
Parathyroid Neoplasms
Parathyroidectomy
Forearm
Adenoma
Effluents
Veins
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Brown, E. M., Brennan, M. F., Broadus, A. R., Marx, S. J., Gardner, D. G., Spiegel, A. M., ... Aurbach, G. D. (1979). Human parathyroid autografts: Comparison of function in vivo and vitro. Journal of Clinical Endocrinology and Metabolism, 48(4), 648-654.

Human parathyroid autografts : Comparison of function in vivo and vitro. / Brown, E. M.; Brennan, M. F.; Broadus, A. R.; Marx, S. J.; Gardner, D. G.; Spiegel, Allen M.; Downs, R. W.; Attie, M. F.; Aurbach, G. D.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 48, No. 4, 1979, p. 648-654.

Research output: Contribution to journalArticle

Brown, EM, Brennan, MF, Broadus, AR, Marx, SJ, Gardner, DG, Spiegel, AM, Downs, RW, Attie, MF & Aurbach, GD 1979, 'Human parathyroid autografts: Comparison of function in vivo and vitro', Journal of Clinical Endocrinology and Metabolism, vol. 48, no. 4, pp. 648-654.
Brown, E. M. ; Brennan, M. F. ; Broadus, A. R. ; Marx, S. J. ; Gardner, D. G. ; Spiegel, Allen M. ; Downs, R. W. ; Attie, M. F. ; Aurbach, G. D. / Human parathyroid autografts : Comparison of function in vivo and vitro. In: Journal of Clinical Endocrinology and Metabolism. 1979 ; Vol. 48, No. 4. pp. 648-654.
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