Human papillomavirus type-16 virus-like particles activate complementary defense responses in key dendritic cell subpopulations

Rongcun Yang, Francisco Martinez Murillo, Ken Yu Lin, William H. Yutzy IV, Satoshi Uematsu, Kiyoshi Takeda, Shizuo Akira, Raphael P. Viscidi, Richard B.S. Roden

Research output: Contribution to journalArticle

15 Scopus citations


Human papillomavirus type-16 (HPV16) L1 virus-like particles (VLPs) activate dendritic cells (DCs) and induce protective immunity. In this study, we demonstrate, using global gene expression analysis, that HPV16 VLPs produce quite distinct innate responses in murine splenic DC subpopulations. While HPV16 VLPs increase transcription of IFN-γ and numerous Th1-related cytokines and chemokines in CD8α+CD11c+ DCs, CD4 +CD11c+ DCs up-regulate only type I IFN and a different set of Th2-associated cytokines and chemokines. Type I IFN, but not IFN-γ, potentiates humoral immunity, notably production of VLP-specific IgG2a. However, HPV16 VLP-stimulated IL-12 production by CD8α +CD11c+ DCs is augmented by autocrine IFN-γ signaling. Thus, before adaptive immunity, HPV16 VLPs signal complementary defense responses in key DC subpopulations, indicating specialized DC lineages with predetermined polarization.

Original languageEnglish (US)
Pages (from-to)2624-2631
Number of pages8
JournalJournal of Immunology
Issue number4
Publication statusPublished - Aug 15 2004
Externally publishedYes


ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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