Human natural killer cells and mature T lymphocytes express identical CD3 ζ subunits as defined by cDNA cloning and sequence analysis

Philippe Moingeon, Christopher C. Stebbins, Lucciano D'Adamio, Jeanne Lucich, Ellis L. Reinherz

Research output: Contribution to journalArticle

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Abstract

In order to characterize the CD3 ζ-related protein found in human natural killer (NK) cells and compare it with CD3 ζ expressed in T lymphocytes, the present study was performed. A polyclonal CD3-CD16+NK population displaying a strong non-major histocompatibility complex-restricted cytotoxic activity against the NK target K-562 was isolated and a product corresponding to CD3 ζ amplified using the polymerase chain reaction method. This 0.6-kb product was present in similar amounts in NK cells and T cells. In contrast, a product corresponding to CD3 δ was amplified from T lymphocytes exclusively. Thus, the CD3 ζ product detected in NK cells did not originate from contaminating T cells. DNA sequence analysis of two independent polymerase chain reaction products from the NK cells demonstrates that human NK cells and mature Tcells share a CD3 ζ subunit with an identical primary amino acid sequence. The nucleotide sequence of a third NK-derived cDNA revealed an insertion of a CAG triplet encoding an additional glutamine residue in the cytoplasmic domain. Since this residue is encoded by nucleotides at a putative RNA splice junction, it possibly results from a difference in pre-mRNA splicing. Taken together, these data show that CD3 ζ is not structurally distinct in NK cells and in T lymphocytes.

Original languageEnglish (US)
Pages (from-to)1741-1745
Number of pages5
JournalEuropean Journal of Immunology
Volume20
Issue number8
StatePublished - Aug 1990
Externally publishedYes

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Natural Killer Cells
Sequence Analysis
Organism Cloning
Complementary DNA
T-Lymphocytes
Polymerase Chain Reaction
RNA Precursors
Major Histocompatibility Complex
Glutamine
DNA Sequence Analysis
Amino Acid Sequence
Nucleotides
RNA
Population
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Human natural killer cells and mature T lymphocytes express identical CD3 ζ subunits as defined by cDNA cloning and sequence analysis. / Moingeon, Philippe; Stebbins, Christopher C.; D'Adamio, Lucciano; Lucich, Jeanne; Reinherz, Ellis L.

In: European Journal of Immunology, Vol. 20, No. 8, 08.1990, p. 1741-1745.

Research output: Contribution to journalArticle

Moingeon, Philippe ; Stebbins, Christopher C. ; D'Adamio, Lucciano ; Lucich, Jeanne ; Reinherz, Ellis L. / Human natural killer cells and mature T lymphocytes express identical CD3 ζ subunits as defined by cDNA cloning and sequence analysis. In: European Journal of Immunology. 1990 ; Vol. 20, No. 8. pp. 1741-1745.
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