Human monoclonal anti-La antibodies. The La protein resides on a subset of Ro particles

M. J. Mamula, E. D. Silverman, R. M. Laxer, L. Bentur, B. Isacovics, J. A. Hardin

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have addressed the problem of anti-La autoimmune responses by defining the specific binding sites of human mAb to the La protein. Two human anti-La mAb were developed; one an IgM(κ) (designated 8G3) and the second an IgG1(κ) (9A5) isotype. The mAb 8G3 immunoprecipitated the La RNA and La protein from crude human cell lysates: bound the 50-kDa La protein and a 28-kDa digestion fragment in immunoblots, and recognized a small defined internal segment from the cloned La protein. In contrast, the IgG isotype (9A5) failed to precipitate native La from cell lysates but bound the same segment of digested La protein and the same polypeptide of 131 amino acids in length from the cloned La protein. Immunoprecipitation experiments performed with these mAb demonstrated that the La protein is a component of a subset of Ro particles. The data suggest that the La protein is not present on the hY RNA in the absence of the Ro polypeptide. These observations may define functional subsets or maturation states of hY RNA based on their association with Ro or Ro and La polypeptides.

Original languageEnglish (US)
Pages (from-to)2923-2928
Number of pages6
JournalJournal of Immunology
Volume143
Issue number9
StatePublished - 1989
Externally publishedYes

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Anti-Idiotypic Antibodies
Monoclonal Antibodies
Proteins
RNA
Peptides
Immunoglobulin G
Autoimmunity
Immunoprecipitation
Immunoglobulin M
Digestion
Binding Sites
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Mamula, M. J., Silverman, E. D., Laxer, R. M., Bentur, L., Isacovics, B., & Hardin, J. A. (1989). Human monoclonal anti-La antibodies. The La protein resides on a subset of Ro particles. Journal of Immunology, 143(9), 2923-2928.

Human monoclonal anti-La antibodies. The La protein resides on a subset of Ro particles. / Mamula, M. J.; Silverman, E. D.; Laxer, R. M.; Bentur, L.; Isacovics, B.; Hardin, J. A.

In: Journal of Immunology, Vol. 143, No. 9, 1989, p. 2923-2928.

Research output: Contribution to journalArticle

Mamula, MJ, Silverman, ED, Laxer, RM, Bentur, L, Isacovics, B & Hardin, JA 1989, 'Human monoclonal anti-La antibodies. The La protein resides on a subset of Ro particles', Journal of Immunology, vol. 143, no. 9, pp. 2923-2928.
Mamula MJ, Silverman ED, Laxer RM, Bentur L, Isacovics B, Hardin JA. Human monoclonal anti-La antibodies. The La protein resides on a subset of Ro particles. Journal of Immunology. 1989;143(9):2923-2928.
Mamula, M. J. ; Silverman, E. D. ; Laxer, R. M. ; Bentur, L. ; Isacovics, B. ; Hardin, J. A. / Human monoclonal anti-La antibodies. The La protein resides on a subset of Ro particles. In: Journal of Immunology. 1989 ; Vol. 143, No. 9. pp. 2923-2928.
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