Human microglia mediate anti-Cryptococcus neoformans activity in the presence of specific antibody

Sunhee C. Lee, Yvonne Kress, Dennis W. Dickson, Arturo Casadevall

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The interaction of the opportunistic fungus Cryptococcus neoformans with human microglia was studied in vitro in the presence and absence of capsule binding antibody. In the absence of capsule binding antibody there was little or no phagocytosis. Addition of the murine monoclonal antibody (mAb) 2H1 (IgG1, kappa) to the capsular glucuronoxylomannan (GXM) produced a dose-dependent enhancement of C. neoformans phagocytosis by microglia. Phagocytosis resulted in marked inhibition of fungal proliferation. Microglial antifungal activity was studied by colony forming unit assay, l-[3H]leucine incorporation assay, and phase contrast microscopy. At microglia: C. neoformans ratios of 10:1 to 80:1 fungal growth was reduced by 61-95%. Inhibitors of nitric oxide synthase and reactive oxygen intermediates did not prevent antifungal activity mediated by human microglia. Transmission electron microscopic studies revealed that although some internalized yeast cells were killed, the majority were intact consistent with fungistasis. Human microglia cells are potent effector cells against C. neoformans in vitro in the presence of specific antibody. Enhancement of microglial activity in vivo by opsonins may be a useful therapeutic strategy.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalJournal of Neuroimmunology
Volume62
Issue number1
DOIs
StatePublished - 1995

Fingerprint

Cryptococcus neoformans
Microglia
Phagocytosis
Antibodies
Capsules
Colony-Forming Units Assay
Opsonin Proteins
Phase-Contrast Microscopy
Human Activities
Nitric Oxide Synthase
Leucine
Fungi
Immunoglobulin G
Yeasts
Monoclonal Antibodies
Electrons
Oxygen
Growth

Keywords

  • AIDS
  • Capsular polysaccharide
  • Cryptococcus neoformans
  • Microglia
  • Monoclonal antibody
  • Phagocytosis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Human microglia mediate anti-Cryptococcus neoformans activity in the presence of specific antibody. / C. Lee, Sunhee; Kress, Yvonne; W. Dickson, Dennis; Casadevall, Arturo.

In: Journal of Neuroimmunology, Vol. 62, No. 1, 1995, p. 43-52.

Research output: Contribution to journalArticle

C. Lee, Sunhee ; Kress, Yvonne ; W. Dickson, Dennis ; Casadevall, Arturo. / Human microglia mediate anti-Cryptococcus neoformans activity in the presence of specific antibody. In: Journal of Neuroimmunology. 1995 ; Vol. 62, No. 1. pp. 43-52.
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