Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2

Janet Rollins, Ingrid Veras, Stephanie Cabarcas, Ian Willis, Laura Schramm

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

RNA polymerase III (RNA pol III) transcribes many of the small structural RNA molecules involved in processing and translation, thereby regulating the growth rate of a cell. Initiation of pol III transcription requires the evolutionarily conserved pol III initiation factor TFIIIB. TFIIIB is the molecular target of regulation by tumor suppressors, including p53, RB and the RB-related pocket proteins. However, our understanding of negative regulation of human TFIIIB-mediated transcription by other proteins is limited. In this study we characterize a RNA pol III luciferase assay and further demonstrate in vivo that a human homolog of yeast Maf1 represses RNA pol III transcription. Additionally, we show that Maf1 repression of RNA pol III transcription occurs via TFIIIB, specifically through the TFIIB family members Brf1 and Brf2.

Original languageEnglish (US)
Pages (from-to)292-302
Number of pages11
JournalInternational Journal of Biological Sciences
Volume3
Issue number5
DOIs
StatePublished - May 1 2007

Keywords

  • Brf2
  • Maf1
  • RNA polymerase III
  • TFIIIB

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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