Human immunodeficiency virus type 1 Nef in human monocyte-like cell line THP-1 expands treg cells via toll-like receptor 2

Xiaolin Qin, Jiahong Yao, Fan Yang, Jiqin Nie, Yanlin Wang, Prof Chaoqi Liu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

CD4 +CD25 + regulatory T cells (Tregs) represent a unique T-cell lineage that is endowed with the ability to actively suppress immune responses in order to inhibit pathogenic damage resulting from over activation of the immune system. In human immunodeficiency virus-1 (HIV-1) infection, suppression of the immune response by Tregs appears to play an opposing role that promotes chronic viral infection. Treg expansion is known as a marker of the severity of HIV infection and as a potential prognostic marker of disease progression. HIV-1 Nef is one of the earliest expressed viral regulatory genes whose expression may play an important role in regulating Treg cells. We established a THP-1 cell line stably expressing HIV-1 Nef and showed that Nef protein was a potent factor for increasing Treg numbers in vitro. We further found that TLR2 plays a critical role in the increase in Treg cells induced by Nef using TLR2-specific siRNA. Our results suggest new strategies for therapeutic and preventive interventions of HIV infection.

Original languageEnglish (US)
Pages (from-to)3515-3524
Number of pages10
JournalJournal of Cellular Biochemistry
Volume112
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Toll-Like Receptor 2
Regulatory T-Lymphocytes
Viruses
HIV-1
Monocytes
T-cells
Cells
Virus Diseases
Cell Line
HIV Infections
nef Gene Products
Viral Genes
Immune system
Cell Lineage
Regulator Genes
Gene expression
Small Interfering RNA
Disease Progression
Immune System
Chemical activation

Keywords

  • HIV-1 Nef
  • SiRNA
  • TLR2
  • Treg cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Human immunodeficiency virus type 1 Nef in human monocyte-like cell line THP-1 expands treg cells via toll-like receptor 2. / Qin, Xiaolin; Yao, Jiahong; Yang, Fan; Nie, Jiqin; Wang, Yanlin; Liu, Prof Chaoqi.

In: Journal of Cellular Biochemistry, Vol. 112, No. 12, 12.2011, p. 3515-3524.

Research output: Contribution to journalArticle

Qin, Xiaolin ; Yao, Jiahong ; Yang, Fan ; Nie, Jiqin ; Wang, Yanlin ; Liu, Prof Chaoqi. / Human immunodeficiency virus type 1 Nef in human monocyte-like cell line THP-1 expands treg cells via toll-like receptor 2. In: Journal of Cellular Biochemistry. 2011 ; Vol. 112, No. 12. pp. 3515-3524.
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