Human immunodeficiency virus type 1 infection of mature CD3(hi)CD8+ thymocytes

Shirley Lee, Harris Goldstein, Michael Baseler, Joe Adelsberger, Hana Golding

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Although CD4+ cells are the primary targets of human immunodeficiency virus type 1 (HIV-1) infection, earlier reports have suggested that intrathymic infection of CD8+ cells may occur. However, it was unclear whether HIV-1-infected CD8+ thymocytes were truly mature single-positive (SP) cells. In the present study, SCID mice implanted with human fetal thymus and liver tissues (SCID-hu mice) were infected with three primary isolates of HIV-1 and infected thymocytes were analyzed to assess maturational status. After intraimplant or intraperitoneal injection with HIV-1, thymocytes were sorted by three-color flow cytometric analysis into mature populations of CD3(hi)CD4+ and CD3(hi)CD8+ SP cells of >99% purity (<0.3% CD4-containing cells in the CD8+ population). The presence of HIV-1 provirus in the sorted thymocyte populations was determined by quantitative PCR. A fraction of mature CD3(hi)CD8+ thymocytes contained HIV-1 proviral DNA, and evidence of viral mRNA transcription in these cells was demonstrated by in situ hybridization. In contrast, when uninfected CD3(hi)CD8+ thymocytes were cocultured with HIV-1-infected CD4+ thymocytes, no evidence of productive HIV-1 infection was detected. Thus, HIV-1 infection of CD8+ thymocytes in the SCID-hu mouse does not occur by direct contact with the virus. Rather, cell surface CD4 is required; therefore, precursor cells are the likely primary target of HIV-1 infection in the thymus. During ontogeny, some of these infected cells continue their differentiation into mature CD8+ SP thymocytes that contain proviral DNA and express viral RNA.

Original languageEnglish (US)
Pages (from-to)6671-6676
Number of pages6
JournalJournal of Virology
Volume71
Issue number9
StatePublished - 1997

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thymocytes
Virus Diseases
Thymocytes
Human immunodeficiency virus 1
HIV-1
infection
SCID Mice
cells
Thymus Gland
mice
Population
proviruses
Proviruses
Viral DNA
Viral RNA
DNA
direct contact
intraperitoneal injection
Intraperitoneal Injections
purity

ASJC Scopus subject areas

  • Immunology

Cite this

Lee, S., Goldstein, H., Baseler, M., Adelsberger, J., & Golding, H. (1997). Human immunodeficiency virus type 1 infection of mature CD3(hi)CD8+ thymocytes. Journal of Virology, 71(9), 6671-6676.

Human immunodeficiency virus type 1 infection of mature CD3(hi)CD8+ thymocytes. / Lee, Shirley; Goldstein, Harris; Baseler, Michael; Adelsberger, Joe; Golding, Hana.

In: Journal of Virology, Vol. 71, No. 9, 1997, p. 6671-6676.

Research output: Contribution to journalArticle

Lee, S, Goldstein, H, Baseler, M, Adelsberger, J & Golding, H 1997, 'Human immunodeficiency virus type 1 infection of mature CD3(hi)CD8+ thymocytes', Journal of Virology, vol. 71, no. 9, pp. 6671-6676.
Lee, Shirley ; Goldstein, Harris ; Baseler, Michael ; Adelsberger, Joe ; Golding, Hana. / Human immunodeficiency virus type 1 infection of mature CD3(hi)CD8+ thymocytes. In: Journal of Virology. 1997 ; Vol. 71, No. 9. pp. 6671-6676.
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