Human immunodeficiency virus-1 principal neutralizing domain peptide-toxin A conjugate vaccine

S. J. Cryz, H. Goldstein, M. Pettoello-Mantovani, A. Kim, E. Fürer, J. U. Que, T. Hasler, A. Rubinstein

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To enhance the potential efficacy of peptide-based vaccines for human immunodeficiency virus-1 (HIV-1), a principal neutralizing domain (PND) peptide (KRIHIGPGRAFYT) (HIV-1MN) was covalently coupled to Pseudomonas aeruginosa toxin A (TA). Immunization of guinea-pigs with this conjugate vaccine, in the absence of an adjuvant, engendered a high-affinity antibody response to the homologous HIV-1MN PND peptide and to analogous peptides from variant strains of HIV-1. A substantial proportion of such antibodies was directed to the conserved GPGRAF motif. Anti-PND peptide antibodies were capable of neutralizing the homologous strain, HIV-1MN, in addition to two heterologous (RF, IIIB) variants, as determined either by inhibition of syncytia formation or by suppression of p24 antigen production in cultured cells. Therefore, the method of conjugation used preserved critical neutralizing epitopes expressed by the PND peptide. Monovalent or polyvalent PND-TA conjugates, which meet all safety criteria for human use, are a promising approach towards the development of an acquired immunodeficiency syndrome (AIDS) vaccine.

Original languageEnglish (US)
Pages (from-to)67-71
Number of pages5
JournalVaccine
Volume13
Issue number1
DOIs
StatePublished - Jan 1995

Keywords

  • Human immunodeficiency virus-1
  • peptide-toxin A conjugate
  • principal neutralizing domain

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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