TY - JOUR
T1 - Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population
AU - Green, Linda
AU - Whittle, Wilbur
AU - Dell'Orco, Robert
AU - Ostrer, Harry
AU - Stein, Gary
AU - Stein, Janet
N1 - Funding Information:
These studies were supported by grants from the Samuel Roberts Noble Foundation, National Science Foundation (PCM-83-18177), NIH (GM-32010) and the March of Dimes Birth Defects Foundation (1-813). We thank Dr Haig Kazazian for providing DNAs from a series of families and Drs Leonard HayRick and Vincent Cristofalo for the WI38 human diploid libroblasts and for the SV40-transformed WI38 cells.
PY - 1986/6
Y1 - 1986/6
N2 - Analysis of the restriction enzyme digests of total genomic DNAs from a broad spectrum of human cell lines and from individuals with different genetic backgrounds, by hybridization with a series of cloned human histone sequences, indicated restriction site polymorphisms (RSPs) for two adjacent human histone genes which reside on chromosome 1. In most cell lines and individuals examined we observed a single 2.05 kb H4 histone Hind III fragment and a 7.0 kb H3 histone Hind III fragment. In contrast, the polymorphisms were manifested as a 2.15 kb H4 Hind III fragment and a 9.1 kb H3 Hind III fragment. From population studies, we were able to show that there is no linkage disequilibrium between these two polymorphic restriction sites. Nor was there any apparent correlation between the presence of the H3/H4 histone polymorphisms and maintenance of the transformed karyotype, passage in culture, transformation or tumor progression. These chromosome 1 H3 and H4 histone gene polymorphisms are common in the American Black population and, in our survey of individuals, were not found in the American Caucasian population. Among the American Blacks studied, the frequency of the H3 Hind III(-) allele is 43 % and of the H4 Hind III(-) allele 30%. In limited family studies, we were unable to detect recombination between these two physically linked alleles.
AB - Analysis of the restriction enzyme digests of total genomic DNAs from a broad spectrum of human cell lines and from individuals with different genetic backgrounds, by hybridization with a series of cloned human histone sequences, indicated restriction site polymorphisms (RSPs) for two adjacent human histone genes which reside on chromosome 1. In most cell lines and individuals examined we observed a single 2.05 kb H4 histone Hind III fragment and a 7.0 kb H3 histone Hind III fragment. In contrast, the polymorphisms were manifested as a 2.15 kb H4 Hind III fragment and a 9.1 kb H3 Hind III fragment. From population studies, we were able to show that there is no linkage disequilibrium between these two polymorphic restriction sites. Nor was there any apparent correlation between the presence of the H3/H4 histone polymorphisms and maintenance of the transformed karyotype, passage in culture, transformation or tumor progression. These chromosome 1 H3 and H4 histone gene polymorphisms are common in the American Black population and, in our survey of individuals, were not found in the American Caucasian population. Among the American Blacks studied, the frequency of the H3 Hind III(-) allele is 43 % and of the H4 Hind III(-) allele 30%. In limited family studies, we were unable to detect recombination between these two physically linked alleles.
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U2 - 10.1016/0014-4827(86)90048-0
DO - 10.1016/0014-4827(86)90048-0
M3 - Article
C2 - 3011475
AN - SCOPUS:0022448212
SN - 0014-4827
VL - 164
SP - 507
EP - 515
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -