Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population

Linda Green, Wilbur Whittle, Robert Dell'Orco, Harry Ostrer, Gary Stein, Janet Stein

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Analysis of the restriction enzyme digests of total genomic DNAs from a broad spectrum of human cell lines and from individuals with different genetic backgrounds, by hybridization with a series of cloned human histone sequences, indicated restriction site polymorphisms (RSPs) for two adjacent human histone genes which reside on chromosome 1. In most cell lines and individuals examined we observed a single 2.05 kb H4 histone Hind III fragment and a 7.0 kb H3 histone Hind III fragment. In contrast, the polymorphisms were manifested as a 2.15 kb H4 Hind III fragment and a 9.1 kb H3 Hind III fragment. From population studies, we were able to show that there is no linkage disequilibrium between these two polymorphic restriction sites. Nor was there any apparent correlation between the presence of the H3/H4 histone polymorphisms and maintenance of the transformed karyotype, passage in culture, transformation or tumor progression. These chromosome 1 H3 and H4 histone gene polymorphisms are common in the American Black population and, in our survey of individuals, were not found in the American Caucasian population. Among the American Blacks studied, the frequency of the H3 Hind III(-) allele is 43 % and of the H4 Hind III(-) allele 30%. In limited family studies, we were unable to detect recombination between these two physically linked alleles.

Original languageEnglish (US)
Pages (from-to)507-515
Number of pages9
JournalExperimental Cell Research
Volume164
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Histones
Population
Genes
Chromosomes, Human, Pair 1
Alleles
Genetic Hybridization
Cell Line
Restriction Mapping
Linkage Disequilibrium
Karyotype
Genetic Recombination
Maintenance
DNA
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population. / Green, Linda; Whittle, Wilbur; Dell'Orco, Robert; Ostrer, Harry; Stein, Gary; Stein, Janet.

In: Experimental Cell Research, Vol. 164, No. 2, 1986, p. 507-515.

Research output: Contribution to journalArticle

Green, Linda ; Whittle, Wilbur ; Dell'Orco, Robert ; Ostrer, Harry ; Stein, Gary ; Stein, Janet. / Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population. In: Experimental Cell Research. 1986 ; Vol. 164, No. 2. pp. 507-515.
@article{49ca3bbdc7ce4f8d81312284f2b5f9b1,
title = "Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population",
abstract = "Analysis of the restriction enzyme digests of total genomic DNAs from a broad spectrum of human cell lines and from individuals with different genetic backgrounds, by hybridization with a series of cloned human histone sequences, indicated restriction site polymorphisms (RSPs) for two adjacent human histone genes which reside on chromosome 1. In most cell lines and individuals examined we observed a single 2.05 kb H4 histone Hind III fragment and a 7.0 kb H3 histone Hind III fragment. In contrast, the polymorphisms were manifested as a 2.15 kb H4 Hind III fragment and a 9.1 kb H3 Hind III fragment. From population studies, we were able to show that there is no linkage disequilibrium between these two polymorphic restriction sites. Nor was there any apparent correlation between the presence of the H3/H4 histone polymorphisms and maintenance of the transformed karyotype, passage in culture, transformation or tumor progression. These chromosome 1 H3 and H4 histone gene polymorphisms are common in the American Black population and, in our survey of individuals, were not found in the American Caucasian population. Among the American Blacks studied, the frequency of the H3 Hind III(-) allele is 43 {\%} and of the H4 Hind III(-) allele 30{\%}. In limited family studies, we were unable to detect recombination between these two physically linked alleles.",
author = "Linda Green and Wilbur Whittle and Robert Dell'Orco and Harry Ostrer and Gary Stein and Janet Stein",
year = "1986",
doi = "10.1016/0014-4827(86)90048-0",
language = "English (US)",
volume = "164",
pages = "507--515",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population

AU - Green, Linda

AU - Whittle, Wilbur

AU - Dell'Orco, Robert

AU - Ostrer, Harry

AU - Stein, Gary

AU - Stein, Janet

PY - 1986

Y1 - 1986

N2 - Analysis of the restriction enzyme digests of total genomic DNAs from a broad spectrum of human cell lines and from individuals with different genetic backgrounds, by hybridization with a series of cloned human histone sequences, indicated restriction site polymorphisms (RSPs) for two adjacent human histone genes which reside on chromosome 1. In most cell lines and individuals examined we observed a single 2.05 kb H4 histone Hind III fragment and a 7.0 kb H3 histone Hind III fragment. In contrast, the polymorphisms were manifested as a 2.15 kb H4 Hind III fragment and a 9.1 kb H3 Hind III fragment. From population studies, we were able to show that there is no linkage disequilibrium between these two polymorphic restriction sites. Nor was there any apparent correlation between the presence of the H3/H4 histone polymorphisms and maintenance of the transformed karyotype, passage in culture, transformation or tumor progression. These chromosome 1 H3 and H4 histone gene polymorphisms are common in the American Black population and, in our survey of individuals, were not found in the American Caucasian population. Among the American Blacks studied, the frequency of the H3 Hind III(-) allele is 43 % and of the H4 Hind III(-) allele 30%. In limited family studies, we were unable to detect recombination between these two physically linked alleles.

AB - Analysis of the restriction enzyme digests of total genomic DNAs from a broad spectrum of human cell lines and from individuals with different genetic backgrounds, by hybridization with a series of cloned human histone sequences, indicated restriction site polymorphisms (RSPs) for two adjacent human histone genes which reside on chromosome 1. In most cell lines and individuals examined we observed a single 2.05 kb H4 histone Hind III fragment and a 7.0 kb H3 histone Hind III fragment. In contrast, the polymorphisms were manifested as a 2.15 kb H4 Hind III fragment and a 9.1 kb H3 Hind III fragment. From population studies, we were able to show that there is no linkage disequilibrium between these two polymorphic restriction sites. Nor was there any apparent correlation between the presence of the H3/H4 histone polymorphisms and maintenance of the transformed karyotype, passage in culture, transformation or tumor progression. These chromosome 1 H3 and H4 histone gene polymorphisms are common in the American Black population and, in our survey of individuals, were not found in the American Caucasian population. Among the American Blacks studied, the frequency of the H3 Hind III(-) allele is 43 % and of the H4 Hind III(-) allele 30%. In limited family studies, we were unable to detect recombination between these two physically linked alleles.

UR - http://www.scopus.com/inward/record.url?scp=0022448212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022448212&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(86)90048-0

DO - 10.1016/0014-4827(86)90048-0

M3 - Article

C2 - 3011475

AN - SCOPUS:0022448212

VL - 164

SP - 507

EP - 515

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -