Human fetal myelinated organotypic cultures

W. D. Lyman, W. C. Hatch, E. Pousada, G. Stephney, W. K. Rashbaum, Karen M. Weidenheim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have previously reported the establishment of organotypic cultures derived from human fetal brain tissue. Although these cultures permit the testing of multiple hypotheses about normal human neurodevelopment and neuropathologic conditions, they have the limitation of not being myelinated and therefore preclude the study of questions related to myelinogenesis and diseases of myelin. In the current communication, we describe recent developments that allow us to overcome this limitation and permit the establishment of a myelinated organotypic culture model. Sections of dorsal column dissected from the lumbar spinal cord of human fetuses ranging in age 21-23 weeks of gestation were placed in culture. The explants were maintained for up to 12 weeks during which time they were characterized and shown to express a number of CNS cell-type-specific markers including glial fibrillary acidic protein (astrocytes), nerve growth factor receptor and neurofilament protein (neurons), CD68 (microglia), and myelin basic protein, HNK-1 and galactocerebroside (oligodendrocytes). In addition, lectin histochemistry using Ricinus communis agglutinin-1 detected microglia and endothelial cells. Upon explantation, abundant myelin was seen by electron microscopy in the cultures. Although during the culture period there was degradation of myelin, there was also evidence of maintenance of intact myelin sheaths around small caliber axons and de novo myelin synthesis. This model system may permit the further use of human organotypic cultures to investigate issues related to neurodevelopment and to pathologic conditions including those relevant to dysmyelination and demyelination.

Original languageEnglish (US)
Pages (from-to)34-44
Number of pages11
JournalBrain Research
Volume599
Issue number1
DOIs
StatePublished - Dec 18 1992

Fingerprint

Myelin Sheath
Microglia
Spinal Cord
Fetus
Neurofilament Proteins
Nerve Growth Factor Receptor
Myelin Basic Protein
Glial Fibrillary Acidic Protein
Oligodendroglia
Demyelinating Diseases
Lectins
Astrocytes
Axons
Electron Microscopy
Endothelial Cells
Cell Count
Communication
Maintenance
Neurons
Pregnancy

Keywords

  • Central nervous system
  • Development
  • Human fetus
  • Myelin
  • Organotypic culture

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Lyman, W. D., Hatch, W. C., Pousada, E., Stephney, G., Rashbaum, W. K., & Weidenheim, K. M. (1992). Human fetal myelinated organotypic cultures. Brain Research, 599(1), 34-44. https://doi.org/10.1016/0006-8993(92)90849-5

Human fetal myelinated organotypic cultures. / Lyman, W. D.; Hatch, W. C.; Pousada, E.; Stephney, G.; Rashbaum, W. K.; Weidenheim, Karen M.

In: Brain Research, Vol. 599, No. 1, 18.12.1992, p. 34-44.

Research output: Contribution to journalArticle

Lyman, WD, Hatch, WC, Pousada, E, Stephney, G, Rashbaum, WK & Weidenheim, KM 1992, 'Human fetal myelinated organotypic cultures', Brain Research, vol. 599, no. 1, pp. 34-44. https://doi.org/10.1016/0006-8993(92)90849-5
Lyman WD, Hatch WC, Pousada E, Stephney G, Rashbaum WK, Weidenheim KM. Human fetal myelinated organotypic cultures. Brain Research. 1992 Dec 18;599(1):34-44. https://doi.org/10.1016/0006-8993(92)90849-5
Lyman, W. D. ; Hatch, W. C. ; Pousada, E. ; Stephney, G. ; Rashbaum, W. K. ; Weidenheim, Karen M. / Human fetal myelinated organotypic cultures. In: Brain Research. 1992 ; Vol. 599, No. 1. pp. 34-44.
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