Human fetal astrocytes induce the expression of blood-brain barrier specific proteins by autologous endothelial cells

A. A. Hurwitz, Joan W. Berman, W. K. Rashbaum, W. D. Lyman

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The blood-brain barrier (BBB) is involved in many normal regulatory mechanisms as well as in pathologic conditions of the central nervous system. Previous studies examining the development and function of the BBB in vitro have primarily utilized cell lines or cultured tissues from non-human sources. In contrast, this study used a coculture system of human fetal astrocytes and autologous endothelial cells. Astrocytes and endothelial cells (EC) were isolated and cultured on the opposite sides of a synthetic permeable membrane. The cocultures were characterized by electron and light microscopy for morphology and by immunocytochemistry for cell-type specific markers. Using these coculture conditions, astrocytes displayed characteristic morphology and expressed glial fibrillary acidic protein. When cocultured with astrocytes, endothelial cells retained factor VIII expression and expressed the BBB-specific proteins, brain-type glucose transporter (GLUT-1) and gamma-glutamyl transpeptidase. This expression was dependent on EC being in close apposition to or in direct contact with astrocytes. The model presented in this study may permit further examination of the role of the BBB in both normal human neurodevelopment and neuropathologic conditions.

Original languageEnglish (US)
Pages (from-to)238-243
Number of pages6
JournalBrain Research
Volume625
Issue number2
DOIs
StatePublished - Oct 22 1993

Fingerprint

Blood-Brain Barrier
Astrocytes
Endothelial Cells
Coculture Techniques
Proteins
Glucose Transporter Type 1
gamma-Glutamyltransferase
Glial Fibrillary Acidic Protein
Factor VIII
Electron Microscopy
Central Nervous System
Immunohistochemistry
Light
Cell Line
Membranes
Brain

Keywords

  • Astrocyte
  • Blood-brain barrier
  • Endothelial cell
  • Human
  • In vitro model

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Human fetal astrocytes induce the expression of blood-brain barrier specific proteins by autologous endothelial cells. / Hurwitz, A. A.; Berman, Joan W.; Rashbaum, W. K.; Lyman, W. D.

In: Brain Research, Vol. 625, No. 2, 22.10.1993, p. 238-243.

Research output: Contribution to journalArticle

@article{dfb132f58ccb40f28cadb2a21fa105f0,
title = "Human fetal astrocytes induce the expression of blood-brain barrier specific proteins by autologous endothelial cells",
abstract = "The blood-brain barrier (BBB) is involved in many normal regulatory mechanisms as well as in pathologic conditions of the central nervous system. Previous studies examining the development and function of the BBB in vitro have primarily utilized cell lines or cultured tissues from non-human sources. In contrast, this study used a coculture system of human fetal astrocytes and autologous endothelial cells. Astrocytes and endothelial cells (EC) were isolated and cultured on the opposite sides of a synthetic permeable membrane. The cocultures were characterized by electron and light microscopy for morphology and by immunocytochemistry for cell-type specific markers. Using these coculture conditions, astrocytes displayed characteristic morphology and expressed glial fibrillary acidic protein. When cocultured with astrocytes, endothelial cells retained factor VIII expression and expressed the BBB-specific proteins, brain-type glucose transporter (GLUT-1) and gamma-glutamyl transpeptidase. This expression was dependent on EC being in close apposition to or in direct contact with astrocytes. The model presented in this study may permit further examination of the role of the BBB in both normal human neurodevelopment and neuropathologic conditions.",
keywords = "Astrocyte, Blood-brain barrier, Endothelial cell, Human, In vitro model",
author = "Hurwitz, {A. A.} and Berman, {Joan W.} and Rashbaum, {W. K.} and Lyman, {W. D.}",
year = "1993",
month = "10",
day = "22",
doi = "10.1016/0006-8993(93)91064-Y",
language = "English (US)",
volume = "625",
pages = "238--243",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Human fetal astrocytes induce the expression of blood-brain barrier specific proteins by autologous endothelial cells

AU - Hurwitz, A. A.

AU - Berman, Joan W.

AU - Rashbaum, W. K.

AU - Lyman, W. D.

PY - 1993/10/22

Y1 - 1993/10/22

N2 - The blood-brain barrier (BBB) is involved in many normal regulatory mechanisms as well as in pathologic conditions of the central nervous system. Previous studies examining the development and function of the BBB in vitro have primarily utilized cell lines or cultured tissues from non-human sources. In contrast, this study used a coculture system of human fetal astrocytes and autologous endothelial cells. Astrocytes and endothelial cells (EC) were isolated and cultured on the opposite sides of a synthetic permeable membrane. The cocultures were characterized by electron and light microscopy for morphology and by immunocytochemistry for cell-type specific markers. Using these coculture conditions, astrocytes displayed characteristic morphology and expressed glial fibrillary acidic protein. When cocultured with astrocytes, endothelial cells retained factor VIII expression and expressed the BBB-specific proteins, brain-type glucose transporter (GLUT-1) and gamma-glutamyl transpeptidase. This expression was dependent on EC being in close apposition to or in direct contact with astrocytes. The model presented in this study may permit further examination of the role of the BBB in both normal human neurodevelopment and neuropathologic conditions.

AB - The blood-brain barrier (BBB) is involved in many normal regulatory mechanisms as well as in pathologic conditions of the central nervous system. Previous studies examining the development and function of the BBB in vitro have primarily utilized cell lines or cultured tissues from non-human sources. In contrast, this study used a coculture system of human fetal astrocytes and autologous endothelial cells. Astrocytes and endothelial cells (EC) were isolated and cultured on the opposite sides of a synthetic permeable membrane. The cocultures were characterized by electron and light microscopy for morphology and by immunocytochemistry for cell-type specific markers. Using these coculture conditions, astrocytes displayed characteristic morphology and expressed glial fibrillary acidic protein. When cocultured with astrocytes, endothelial cells retained factor VIII expression and expressed the BBB-specific proteins, brain-type glucose transporter (GLUT-1) and gamma-glutamyl transpeptidase. This expression was dependent on EC being in close apposition to or in direct contact with astrocytes. The model presented in this study may permit further examination of the role of the BBB in both normal human neurodevelopment and neuropathologic conditions.

KW - Astrocyte

KW - Blood-brain barrier

KW - Endothelial cell

KW - Human

KW - In vitro model

UR - http://www.scopus.com/inward/record.url?scp=0027373799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027373799&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(93)91064-Y

DO - 10.1016/0006-8993(93)91064-Y

M3 - Article

VL - 625

SP - 238

EP - 243

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -