Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery

Enko N. Kiprilov, Aashir Awan, Romain Desprat, Michelle Velho, Christian A. Clement, Anne Grete Byskov, Claus Y. Andersen, Peter Satir, Eric E. Bouhassira, Søren T. Christensen, Rhoda Elison Hirsch

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Human embryonic stem cells (hESCs) are potential therapeutic tools and models of human development. With a growing interest in primary cilia in signal transduction pathways that are crucial for embryological development and tissue differentiation and interest in mechanisms regulating human hESC differentiation, demonstrating the existence of primary cilia and the localization of signaling components in undifferentiated hESCs establishes a mechanistic basis for the regulation of hESC differentiation. Using electron microscopy (EM), immunofluorescence, and confocal microscopies, we show that primary cilia are present in three undifferentiated hESC lines. EM reveals the characteristic 9 + 0 axoneme. The number and length of cilia increase after serum starvation. Important components of the hedgehog (Hh) pathway, including smoothened, patched 1 (Ptc1), and Gli1 and 2, are present in the cilia. Stimulation of the pathway results in the concerted movement of Ptc1 out of, and smoothened into, the primary cilium as well as up-regulation of GLI1 and PTC1. These findings show that hESCs contain primary cilia associated with working Hh machinery.

Original languageEnglish (US)
Pages (from-to)897-904
Number of pages8
JournalJournal of Cell Biology
Volume180
Issue number5
DOIs
StatePublished - Mar 10 2008

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Cilia
Cell Culture Techniques
Cell Differentiation
Electron Microscopy
Axoneme
Human Development
Starvation
Human Embryonic Stem Cells
Fluorescence Microscopy
Confocal Microscopy
Signal Transduction
Up-Regulation
Cell Line
Serum

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kiprilov, E. N., Awan, A., Desprat, R., Velho, M., Clement, C. A., Byskov, A. G., ... Hirsch, R. E. (2008). Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. Journal of Cell Biology, 180(5), 897-904. https://doi.org/10.1083/jcb.200706028

Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. / Kiprilov, Enko N.; Awan, Aashir; Desprat, Romain; Velho, Michelle; Clement, Christian A.; Byskov, Anne Grete; Andersen, Claus Y.; Satir, Peter; Bouhassira, Eric E.; Christensen, Søren T.; Hirsch, Rhoda Elison.

In: Journal of Cell Biology, Vol. 180, No. 5, 10.03.2008, p. 897-904.

Research output: Contribution to journalArticle

Kiprilov, EN, Awan, A, Desprat, R, Velho, M, Clement, CA, Byskov, AG, Andersen, CY, Satir, P, Bouhassira, EE, Christensen, ST & Hirsch, RE 2008, 'Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery', Journal of Cell Biology, vol. 180, no. 5, pp. 897-904. https://doi.org/10.1083/jcb.200706028
Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG et al. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. Journal of Cell Biology. 2008 Mar 10;180(5):897-904. https://doi.org/10.1083/jcb.200706028
Kiprilov, Enko N. ; Awan, Aashir ; Desprat, Romain ; Velho, Michelle ; Clement, Christian A. ; Byskov, Anne Grete ; Andersen, Claus Y. ; Satir, Peter ; Bouhassira, Eric E. ; Christensen, Søren T. ; Hirsch, Rhoda Elison. / Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. In: Journal of Cell Biology. 2008 ; Vol. 180, No. 5. pp. 897-904.
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