Human colony-stimulating factor (CSF-1) radioimmunoassay: Resolution of three subclasses of human colony-stimulating factors

S. K. Das, E. R. Stanley, L. J. Guilbert, L. W. Forman

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

The partial purification of human urinary CSF-1 to approximately 4 x 106 U/mg protein from large volumes of human urine is described. Carrier-free iodination of the partially purified preparation and subsequent receptor binding and elution of the resulting 125I-CSF-1 yielded 125I-CSF-1, which, by several criteria, was free of contaminating 125I-proteins. The 125I-CSF-1 (approximately 4 x 107 U/mg of iodinated protein) was biologically active and suitable for use in target cell binding studies. It was used to develop a RIA that specifically detects the human CSF subclass that stimulates macrophage production (CSF-1). This RIA, the CSF-1 radioreceptor assay, and the murine and human CSF bioassays were used in comparative studies of a variety of CSF preparations. Results of these studies indicate that there are at least three human CSF subclasses: CSF-1 which stimulates the formation of colonies containing macrophages by murine and human hemopoietic cells; another subclass stimulating the formation of colonies (possibly containing neutrophils, eosinophils, and macrophages) by human but not murine hemopoietic cells; and a third subclass that stimulates the formation of colonies (possibly containing neutrophils and macrophages) by both human and murine hemopoietic cells. Anti-human urinary CSF-1 antibody selectively prevented the development of human bone marrow colonies with the gross morphology of those stimulated by purified human urinary CSF-1, in cultures stimulated by a variety of CSF preparations. The average level of CSF-1 in the serum of 14 normal adult humans, as determined by RIA, was 174 ± 76 U/ml (approximately 40 pM).

Original languageEnglish (US)
Pages (from-to)630-641
Number of pages12
JournalBlood
Volume58
Issue number3
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Human colony-stimulating factor (CSF-1) radioimmunoassay: Resolution of three subclasses of human colony-stimulating factors'. Together they form a unique fingerprint.

  • Cite this

    Das, S. K., Stanley, E. R., Guilbert, L. J., & Forman, L. W. (1981). Human colony-stimulating factor (CSF-1) radioimmunoassay: Resolution of three subclasses of human colony-stimulating factors. Blood, 58(3), 630-641.