Abstract
We have previously shown that highly purified urinary hCG has the potential to both stimulate the intracellular accumulation of cyclic AMP and induce growth of immortalized rat thyroid cells. We have now compared the ability of recombinant human TSH and purified urinary hCG preparations to stimulate Chinese hamster ovary (CHO) cells which have been transfected with the human TSH receptor. Only transfected CHO cells expressing recombinant TSH receptors, but not control CHO cells, were stimulated by hCG to release cyclic AMP in a dose-related manner and the effect of 100 IU of HCG was equivalent to approximately 9.2 uU of rec-hTSH. These data demonstrate that hCG interacts directly with the human TSH receptor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1477-1479 |
| Number of pages | 3 |
| Journal | Journal of Clinical Endocrinology and Metabolism |
| Volume | 74 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Endocrinology, Diabetes and Metabolism
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Human chorionic gonadotropin (hCG) interacts directly with recombinant human TSH receptors. / Tomer, Yaron; Huber, G. K.; Davies, T. F.
In: Journal of Clinical Endocrinology and Metabolism, Vol. 74, No. 6, 1992, p. 1477-1479.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human chorionic gonadotropin (hCG) interacts directly with recombinant human TSH receptors
AU - Tomer, Yaron
AU - Huber, G. K.
AU - Davies, T. F.
PY - 1992
Y1 - 1992
N2 - We have previously shown that highly purified urinary hCG has the potential to both stimulate the intracellular accumulation of cyclic AMP and induce growth of immortalized rat thyroid cells. We have now compared the ability of recombinant human TSH and purified urinary hCG preparations to stimulate Chinese hamster ovary (CHO) cells which have been transfected with the human TSH receptor. Only transfected CHO cells expressing recombinant TSH receptors, but not control CHO cells, were stimulated by hCG to release cyclic AMP in a dose-related manner and the effect of 100 IU of HCG was equivalent to approximately 9.2 uU of rec-hTSH. These data demonstrate that hCG interacts directly with the human TSH receptor.
AB - We have previously shown that highly purified urinary hCG has the potential to both stimulate the intracellular accumulation of cyclic AMP and induce growth of immortalized rat thyroid cells. We have now compared the ability of recombinant human TSH and purified urinary hCG preparations to stimulate Chinese hamster ovary (CHO) cells which have been transfected with the human TSH receptor. Only transfected CHO cells expressing recombinant TSH receptors, but not control CHO cells, were stimulated by hCG to release cyclic AMP in a dose-related manner and the effect of 100 IU of HCG was equivalent to approximately 9.2 uU of rec-hTSH. These data demonstrate that hCG interacts directly with the human TSH receptor.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026750460&partnerID=8YFLogxK
U2 - 10.1210/jc.74.6.1477
DO - 10.1210/jc.74.6.1477
M3 - Article
VL - 74
SP - 1477
EP - 1479
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 6
ER -