Human Cep192 Is Required for Mitotic Centrosome and Spindle Assembly

Maria Ana Gomez-Ferreria, Uttama Rath, Daniel W. Buster, Sumit K. Chanda, Jeremy S. Caldwell, Daniel R. Rines, David J. Sharp

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

As cells enter mitosis, centrosomes dramatically increase in size and ability to nucleate microtubules. This process, termed centrosome maturation, is driven by the accumulation and activation of γ-tubulin and other proteins that form the pericentriolar material on centrosomes during G2/prophase. Here, we show that the human centrosomal protein, Cep192 (centrosomal protein of 192 kDa), is an essential component of the maturation machinery. Specifically, we have found that siRNA depletion of Cep192 results in a complete loss of functional centrosomes in mitotic but not interphase cells. In mitotic cells lacking Cep192, microtubules become organized around chromosomes but rarely acquire stable bipolar configurations. These cells contain normal numbers of centrioles but cannot assemble γ-tubulin, pericentrin, or other pericentriolar proteins into an organized PCM. Alternatively, overexpression of Cep192 results in the formation of multiple, extracentriolar foci of γ-tubulin and pericentrin. Together, our findings support the hypothesis that Cep192 stimulates the formation of the scaffolding upon which γ-tubulin ring complexes and other proteins involved in microtubule nucleation and spindle assembly become functional during mitosis.

Original languageEnglish (US)
Pages (from-to)1960-1966
Number of pages7
JournalCurrent Biology
Volume17
Issue number22
DOIs
StatePublished - Nov 20 2007

Fingerprint

centrosomes
Centrosome
Spindle Apparatus
tubulin
Tubulin
Proteins
proteins
microtubules
Microtubules
mitosis
Mitosis
material forms
centrioles
Centrioles
protein depletion
Prophase
prophase
cells
Pulse code modulation
small interfering RNA

Keywords

  • CELLCYCLE

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Gomez-Ferreria, M. A., Rath, U., Buster, D. W., Chanda, S. K., Caldwell, J. S., Rines, D. R., & Sharp, D. J. (2007). Human Cep192 Is Required for Mitotic Centrosome and Spindle Assembly. Current Biology, 17(22), 1960-1966. https://doi.org/10.1016/j.cub.2007.10.019

Human Cep192 Is Required for Mitotic Centrosome and Spindle Assembly. / Gomez-Ferreria, Maria Ana; Rath, Uttama; Buster, Daniel W.; Chanda, Sumit K.; Caldwell, Jeremy S.; Rines, Daniel R.; Sharp, David J.

In: Current Biology, Vol. 17, No. 22, 20.11.2007, p. 1960-1966.

Research output: Contribution to journalArticle

Gomez-Ferreria, MA, Rath, U, Buster, DW, Chanda, SK, Caldwell, JS, Rines, DR & Sharp, DJ 2007, 'Human Cep192 Is Required for Mitotic Centrosome and Spindle Assembly', Current Biology, vol. 17, no. 22, pp. 1960-1966. https://doi.org/10.1016/j.cub.2007.10.019
Gomez-Ferreria MA, Rath U, Buster DW, Chanda SK, Caldwell JS, Rines DR et al. Human Cep192 Is Required for Mitotic Centrosome and Spindle Assembly. Current Biology. 2007 Nov 20;17(22):1960-1966. https://doi.org/10.1016/j.cub.2007.10.019
Gomez-Ferreria, Maria Ana ; Rath, Uttama ; Buster, Daniel W. ; Chanda, Sumit K. ; Caldwell, Jeremy S. ; Rines, Daniel R. ; Sharp, David J. / Human Cep192 Is Required for Mitotic Centrosome and Spindle Assembly. In: Current Biology. 2007 ; Vol. 17, No. 22. pp. 1960-1966.
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