Human cardiac stem cell differentiation is regulated by a mircrine mechanism

Toru Hosoda, Hanqiao Zheng, Mauricio Cabral-Da-Silva, Fumihiro Sanada, Noriko Ide-Iwata, Barbara Ogórek, João Ferreira-Martins, Christian Arranto, Domenico D'Amario, Federica Del Monte, Konrad Urbanek, David A. D'Alessandro, Robert E. Michler, Piero Anversa, Marcello Rota, Jan Kajstura, Annarosa Leri

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Background- Cardiac stem cells (CSCs) delivered to the infarcted heart generate a large number of small fetal-neonatal cardiomyocytes that fail to acquire the differentiated phenotype. However, the interaction of CSCs with postmitotic myocytes results in the formation of cells with adult characteristics. Methods and Results- On the basis of results of in vitro and in vivo assays, we report that the commitment of human CSCs (hCSCs) to the myocyte lineage and the generation of mature working cardiomyocytes are influenced by microRNA-499 (miR-499), which is barely detectable in hCSCs but is highly expressed in postmitotic human cardiomyocytes. miR-499 traverses gap junction channels and translocates to structurally coupled hCSCs favoring their differentiation into functionally competent cells. Expression of miR-499 in hCSCs represses the miR-499 target genes Sox6 and Rod1, enhancing cardiomyogenesis in vitro and after infarction in vivo. Although cardiac repair was detected in all cell-treated infarcted hearts, the aggregate volume of the regenerated myocyte mass and myocyte cell volume were greater in animals injected with hCSCs overexpressing miR-499. Treatment with hCSCs resulted in an improvement in ventricular function, consisting of a better preservation of developed pressure and positive and negative dP/dt after infarction. An additional positive effect on cardiac performance occurred with miR-499, pointing to enhanced myocyte differentiation/hypertrophy as the mechanism by which miR-499 potentiated the restoration of myocardial mass and function in the infarcted heart. Conclusions- The recognition that miR-499 promotes the differentiation of hCSCs into mechanically integrated cardiomyocytes has important clinical implications for the treatment of human heart failure.

Original languageEnglish (US)
Pages (from-to)1287-1296
Number of pages10
JournalCirculation
Volume123
Issue number12
DOIs
StatePublished - Mar 29 2011

Fingerprint

MicroRNAs
Cell Differentiation
Stem Cells
Muscle Cells
Cardiac Myocytes
Infarction
Cardiac Volume
Ventricular Function
Gap Junctions
Cell Size
Hypertrophy
Heart Failure
Phenotype
Pressure
Genes

Keywords

  • cardiomyogenesis
  • gap junctions
  • microRNA
  • mircrine
  • stem cells

ASJC Scopus subject areas

  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Hosoda, T., Zheng, H., Cabral-Da-Silva, M., Sanada, F., Ide-Iwata, N., Ogórek, B., ... Leri, A. (2011). Human cardiac stem cell differentiation is regulated by a mircrine mechanism. Circulation, 123(12), 1287-1296. https://doi.org/10.1161/CIRCULATIONAHA.110.982918

Human cardiac stem cell differentiation is regulated by a mircrine mechanism. / Hosoda, Toru; Zheng, Hanqiao; Cabral-Da-Silva, Mauricio; Sanada, Fumihiro; Ide-Iwata, Noriko; Ogórek, Barbara; Ferreira-Martins, João; Arranto, Christian; D'Amario, Domenico; Del Monte, Federica; Urbanek, Konrad; D'Alessandro, David A.; Michler, Robert E.; Anversa, Piero; Rota, Marcello; Kajstura, Jan; Leri, Annarosa.

In: Circulation, Vol. 123, No. 12, 29.03.2011, p. 1287-1296.

Research output: Contribution to journalArticle

Hosoda, T, Zheng, H, Cabral-Da-Silva, M, Sanada, F, Ide-Iwata, N, Ogórek, B, Ferreira-Martins, J, Arranto, C, D'Amario, D, Del Monte, F, Urbanek, K, D'Alessandro, DA, Michler, RE, Anversa, P, Rota, M, Kajstura, J & Leri, A 2011, 'Human cardiac stem cell differentiation is regulated by a mircrine mechanism', Circulation, vol. 123, no. 12, pp. 1287-1296. https://doi.org/10.1161/CIRCULATIONAHA.110.982918
Hosoda T, Zheng H, Cabral-Da-Silva M, Sanada F, Ide-Iwata N, Ogórek B et al. Human cardiac stem cell differentiation is regulated by a mircrine mechanism. Circulation. 2011 Mar 29;123(12):1287-1296. https://doi.org/10.1161/CIRCULATIONAHA.110.982918
Hosoda, Toru ; Zheng, Hanqiao ; Cabral-Da-Silva, Mauricio ; Sanada, Fumihiro ; Ide-Iwata, Noriko ; Ogórek, Barbara ; Ferreira-Martins, João ; Arranto, Christian ; D'Amario, Domenico ; Del Monte, Federica ; Urbanek, Konrad ; D'Alessandro, David A. ; Michler, Robert E. ; Anversa, Piero ; Rota, Marcello ; Kajstura, Jan ; Leri, Annarosa. / Human cardiac stem cell differentiation is regulated by a mircrine mechanism. In: Circulation. 2011 ; Vol. 123, No. 12. pp. 1287-1296.
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AU - Zheng, Hanqiao

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AU - Ide-Iwata, Noriko

AU - Ogórek, Barbara

AU - Ferreira-Martins, João

AU - Arranto, Christian

AU - D'Amario, Domenico

AU - Del Monte, Federica

AU - Urbanek, Konrad

AU - D'Alessandro, David A.

AU - Michler, Robert E.

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