Human cardiac sodium channels expressed in Xenopus oocytes

Gordon F. Tomaselli, A. M. Feldman, G. Yellen, E. Marban

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We report the expression of voltage-dependent Na+ channels in Xenopus oocytes injected with total RNA isolated from explanted human hearts. The expressed channels demonstrate characteristic voltage-dependent gating, inhibition by tetrodotoxin, and selectivity for Na+. Oocytes injected with sterile water or intentionally degraded RNA had no similar channel activity. The antiarrhythmic agent lidocaine (20 μM) inhibits current flow through the channel in a voltage-dependent fashion. Na+ channels expressed by injection of human cardiac RNA into Xenopus oocytes qualitatively resemble channels in the native tissue.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume258
Issue number3 27-3
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Sodium Channels
Xenopus
Oocytes
RNA
Tetrodotoxin
Lidocaine
Injections
Water

Keywords

  • antiarrhythmic drugs, tetrodotoxin
  • heterologous expression
  • ion channels
  • lidocaine
  • sodium current

ASJC Scopus subject areas

  • Physiology

Cite this

Human cardiac sodium channels expressed in Xenopus oocytes. / Tomaselli, Gordon F.; Feldman, A. M.; Yellen, G.; Marban, E.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 258, No. 3 27-3, 01.01.1990.

Research output: Contribution to journalArticle

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