Human cardiac sodium channels expressed in Xenopus oocytes

G. F. Tomaselli, A. M. Feldman, G. Yellen, E. Marban

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We report the expression of voltage-dependent Na+ channels in Xenopus oocytes injected with total RNA isolated from explanted human hearts. The expressed channels demonstrate characteristic voltage-dependent gating, inhibition by tetrodotoxin, and selectivity for Na+. Oocytes injected with sterile water or intentionally degraded RNA had no similar channel activity. The antiarrhythmic agent lidocaine (20 μM) inhibits current flow through the channel in a voltage-dependent fashion. Na+ channels expressed by injection of human cardiac RNA into Xenopus oocytes qualitatively resemble channels in the native tissue.

Original languageEnglish (US)
Pages (from-to)H903-H906
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume258
Issue number3 27-3
DOIs
StatePublished - 1990

Keywords

  • antiarrhythmic drugs, tetrodotoxin
  • heterologous expression
  • ion channels
  • lidocaine
  • sodium current

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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