Human brain β-hydroxybutyrate and lactate increase in fasting-induced ketosis

Jullie W. Pan, Douglas L. Rothman, Kevin L. Behar, Daniel T. Stein, Hoby P. Hetherington

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Ketones are known to constitute an important fraction of fuel for consumption by the brain, with brain ketone content generally thought to be low. However, the recent observation of 1-mmol/L levels of brain β-hydroxybutyrate (BHB) in children on the ketogenic diet suggests otherwise. The authors report the measurement of brain BHB and lactate in the occipital lobe of healthy adults using high field (4-T) magnetic resonance spectroscopy, measured in the nonfasted state and after 2- and 3-day fasting-induced ketosis. A 9-mL voxel located in the calcarine fissure was studied, detecting the BHB and lactate upfield resonances using a 1H homonuclear editing sequence. Plasma BHB levels also were measured. The mean brain BHB concentration increased from a nonfasted level of 0.05 ± 0.05 to 0.60 ± 0.26 mmol/L (after second day of fasting), increasing further to 0.98 ± 0.16 mmol/L (after the third day of fasting). The mean nonfasted brain lactate was 0.69 ± 0.17 mmol/L, increasing to 1.47 ± 0.22 mmol/L after the third day. The plasma and brain BHB levels correlated well (r = 0.86) with a brain-plasma slope of 0.26. These data show that brain BHB rises significantly with 2- and 3-day fasting-induced ketosis. The lactate increase likely results from ketones displacing lactate oxidation without altering glucose phosphorylation and glycolysis.

Original languageEnglish (US)
Pages (from-to)1502-1507
Number of pages6
JournalJournal of Cerebral Blood Flow and Metabolism
Volume20
Issue number10
StatePublished - 2000

Fingerprint

Hydroxybutyrates
Ketosis
Lactic Acid
Fasting
Brain
Ketones
Occipital Lobe
Ketogenic Diet
Glycolysis

Keywords

  • Brain metabolism
  • Energetics
  • Fasting
  • Ketone body
  • Lactate

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Pan, J. W., Rothman, D. L., Behar, K. L., Stein, D. T., & Hetherington, H. P. (2000). Human brain β-hydroxybutyrate and lactate increase in fasting-induced ketosis. Journal of Cerebral Blood Flow and Metabolism, 20(10), 1502-1507.

Human brain β-hydroxybutyrate and lactate increase in fasting-induced ketosis. / Pan, Jullie W.; Rothman, Douglas L.; Behar, Kevin L.; Stein, Daniel T.; Hetherington, Hoby P.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 20, No. 10, 2000, p. 1502-1507.

Research output: Contribution to journalArticle

Pan, JW, Rothman, DL, Behar, KL, Stein, DT & Hetherington, HP 2000, 'Human brain β-hydroxybutyrate and lactate increase in fasting-induced ketosis', Journal of Cerebral Blood Flow and Metabolism, vol. 20, no. 10, pp. 1502-1507.
Pan, Jullie W. ; Rothman, Douglas L. ; Behar, Kevin L. ; Stein, Daniel T. ; Hetherington, Hoby P. / Human brain β-hydroxybutyrate and lactate increase in fasting-induced ketosis. In: Journal of Cerebral Blood Flow and Metabolism. 2000 ; Vol. 20, No. 10. pp. 1502-1507.
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