Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells

Louis Z. Sharp, Kosaku Shinoda, Haruya Ohno, David W. Scheel, Emi Tomoda, Lauren Ruiz, Houchun Hu, Larry Wang, Zdena Pavlova, Vicente Gilsanz, Shingo Kajimura

Research output: Contribution to journalArticle

365 Citations (Scopus)

Abstract

Brown adipose tissue (BAT) dissipates chemical energy and generates heat to protect animals from cold and obesity. Rodents possess two types of UCP-1 positive brown adipocytes arising from distinct developmental lineages: "classical" brown adipocytes develop during the prenatal stage whereas "beige" or "brite" cells that reside in white adipose tissue (WAT) develop during the postnatal stage in response to chronic cold or PPARγ agonists. Beige cells' inducible characteristics make them a promising therapeutic target for obesity treatment, however, the relevance of this cell type in humans remains unknown. In the present study, we determined the gene signatures that were unique to classical brown adipocytes and to beige cells induced by a specific PPARγ agonist rosiglitazone in mice. Subsequently we applied the transcriptional data to humans and examined the molecular signatures of human BAT isolated from multiple adipose depots. To our surprise, nearly all the human BAT abundantly expressed beige cell-selective genes, but the expression of classical brown fat-selective genes were nearly undetectable. Interestingly, expression of known brown fat-selective genes such as PRDM16 was strongly correlated with that of the newly identified beige cell-selective genes, but not with that of classical brown fat-selective genes. Furthermore, histological analyses showed that a new beige cell marker, CITED1, was selectively expressed in the UCP1-positive beige cells as well as in human BAT. These data indicate that human BAT may be primary composed of beige/brite cells.

Original languageEnglish (US)
Article numbere49452
JournalPLoS One
Volume7
Issue number11
DOIs
StatePublished - Nov 16 2012
Externally publishedYes

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Brown Adipose Tissue
brown adipose tissue
Genes
Tissue
Brown Adipocytes
Peroxisome Proliferator-Activated Receptors
rosiglitazone
Fats
cells
adipocytes
genes
agonists
Obesity
obesity
White Adipose Tissue
Beige Adipocytes
white adipose tissue
Animals
Rodentia
Hot Temperature

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sharp, L. Z., Shinoda, K., Ohno, H., Scheel, D. W., Tomoda, E., Ruiz, L., ... Kajimura, S. (2012). Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells. PLoS One, 7(11), [e49452]. https://doi.org/10.1371/journal.pone.0049452

Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells. / Sharp, Louis Z.; Shinoda, Kosaku; Ohno, Haruya; Scheel, David W.; Tomoda, Emi; Ruiz, Lauren; Hu, Houchun; Wang, Larry; Pavlova, Zdena; Gilsanz, Vicente; Kajimura, Shingo.

In: PLoS One, Vol. 7, No. 11, e49452, 16.11.2012.

Research output: Contribution to journalArticle

Sharp, LZ, Shinoda, K, Ohno, H, Scheel, DW, Tomoda, E, Ruiz, L, Hu, H, Wang, L, Pavlova, Z, Gilsanz, V & Kajimura, S 2012, 'Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells', PLoS One, vol. 7, no. 11, e49452. https://doi.org/10.1371/journal.pone.0049452
Sharp, Louis Z. ; Shinoda, Kosaku ; Ohno, Haruya ; Scheel, David W. ; Tomoda, Emi ; Ruiz, Lauren ; Hu, Houchun ; Wang, Larry ; Pavlova, Zdena ; Gilsanz, Vicente ; Kajimura, Shingo. / Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells. In: PLoS One. 2012 ; Vol. 7, No. 11.
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