Human Barrett's adenocarcinoma of the esophagus, associated myofibroblasts, and endothelium can arise from bone marrow-derived cells after allogeneic stem cell transplant

Lloyd Hutchinson, Bjorn Stenstrom, Duan Chen, Bilal Piperdi, Sara Levey, Stephen Lyle, Timothy C. Wang, Jeanmarie Houghton

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

This study characterizes the contribution of bone marrow-derived cells (BMDCs) to Barrett's adenocarcinoma of the esophagus using a mouse surgical model of disease and human specimens. Transplantation of bone marrow expressing beta galactosidase into a wild-type mouse, followed by surgical esophagojejunostomy, allowed tracking of BMDCs into the surgical anastomosis and resulting Barrett's metaplasia. Human tissue from a male patient who had been transplanted with female bone marrow and later developed esophageal adenocarcinoma allowed us to tract donor-derived cells into the tumor. Using a combination of antibodies directed against beta-galactosidase (animal studies) and X/Y fluorescent in situ hybridization (FISH) (human studies), combined with specific lineage staining directed against epithelial, fibroblast, endothelial, and leukocyte markers, we show that bone marrow cells contribute to both the epithelial and stromal component of esophageal adenocarcinoma. These findings demonstrate that BMDCs can generate cancer-associated fibroblasts as well as contribute directly to epithelial cells in cancer of the esophagus.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalStem Cells and Development
Volume20
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

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Barrett Esophagus
Myofibroblasts
Bone Marrow Cells
Endothelium
Stem Cells
Transplants
beta-Galactosidase
Adenocarcinoma
Surgical Anastomosis
Anatomic Models
Esophageal Neoplasms
Fluorescence In Situ Hybridization
Bone Marrow Transplantation
Leukocytes
Fibroblasts
Bone Marrow
Epithelial Cells
Tissue Donors
Staining and Labeling
Adenocarcinoma Of Esophagus

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Human Barrett's adenocarcinoma of the esophagus, associated myofibroblasts, and endothelium can arise from bone marrow-derived cells after allogeneic stem cell transplant. / Hutchinson, Lloyd; Stenstrom, Bjorn; Chen, Duan; Piperdi, Bilal; Levey, Sara; Lyle, Stephen; Wang, Timothy C.; Houghton, Jeanmarie.

In: Stem Cells and Development, Vol. 20, No. 1, 01.01.2011, p. 11-17.

Research output: Contribution to journalArticle

Hutchinson, Lloyd ; Stenstrom, Bjorn ; Chen, Duan ; Piperdi, Bilal ; Levey, Sara ; Lyle, Stephen ; Wang, Timothy C. ; Houghton, Jeanmarie. / Human Barrett's adenocarcinoma of the esophagus, associated myofibroblasts, and endothelium can arise from bone marrow-derived cells after allogeneic stem cell transplant. In: Stem Cells and Development. 2011 ; Vol. 20, No. 1. pp. 11-17.
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