Human autoantibodies recognizing a native macromolecular structure composed of Sm core proteins in U small nuclear rnp particles

Yoshihiko Takeda, Kim S. Wise, Grace Wang, Giuia Grady, Evelyn V. Hess, Gordon C. Sharp, William S. Dynan, John A. Hardin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective. Monoclonal antibody (mAb) F78 recognizes a heat-labile particle composed of Sm core proteins designated F78P. The objective of this study was to identify human autoantibodies recognizing the conformational structure of F78P. Methods. Immunoblots using HeLa cell extracts without heating prior to sodium dodecyl sulfatepolyacrylamide gel electrophoresis were used to identify autoantibodies recognizing F78P. To confirm reactivities with F78P, immunoprecipitates of mAb F78 were used as a substrate for immunoblots. To identify reactivities against the F78P structure in classic anti-Sm-positive sera, autoantibodies to individual Sm core proteins were absorbed with purified U1 small nuclear RNP before immunoblotting. Results. We identified 2 sera that, like F78, recognized only F78P and not its component polypeptides. When classic anti-Sm antibodies were preabsorbed, the presence of F78-1ike, particle-specific antibodies was revealed in all of the anti-Sm-positive sera tested. Conclusion. Autoantibodies against the F78P structure were commonly present in sera from patients with systemic rheumatic diseases, often in combination with anti-Sm autoantibodies.

Original languageEnglish (US)
Pages (from-to)2059-2067
Number of pages9
JournalArthritis and Rheumatism
Volume41
Issue number11
DOIs
StatePublished - 1998
Externally publishedYes

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snRNP Core Proteins
Autoantibodies
Serum
Monoclonal Antibodies
Small Nuclear Ribonucleoproteins
Rheumatic Diseases
Cell Extracts
HeLa Cells
Immunoblotting
Heating
Electrophoresis
Anti-Idiotypic Antibodies
Hot Temperature
Gels
Sodium
Peptides
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Human autoantibodies recognizing a native macromolecular structure composed of Sm core proteins in U small nuclear rnp particles. / Takeda, Yoshihiko; Wise, Kim S.; Wang, Grace; Grady, Giuia; Hess, Evelyn V.; Sharp, Gordon C.; Dynan, William S.; Hardin, John A.

In: Arthritis and Rheumatism, Vol. 41, No. 11, 1998, p. 2059-2067.

Research output: Contribution to journalArticle

Takeda, Yoshihiko ; Wise, Kim S. ; Wang, Grace ; Grady, Giuia ; Hess, Evelyn V. ; Sharp, Gordon C. ; Dynan, William S. ; Hardin, John A. / Human autoantibodies recognizing a native macromolecular structure composed of Sm core proteins in U small nuclear rnp particles. In: Arthritis and Rheumatism. 1998 ; Vol. 41, No. 11. pp. 2059-2067.
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AU - Hess, Evelyn V.

AU - Sharp, Gordon C.

AU - Dynan, William S.

AU - Hardin, John A.

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