Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles: The effect on neuron-like differentiation in vitro

Guangwei Zeng, Gongping Wang, Fangxia Guan, Keliang Chang, Hongliang Jiao, Wanqin Gao, Shoumin Xi, Bo Yang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) have the potential for self-renewal and multipotential differentiation to regenerate damaged tissues or recover functional absence in diseases. Superparamagnetic iron oxide nano-particles (SPIONs) are used as contrast agents in magnetic resonance imaging (MRI) for labeling cells in vitro and for tracking SPION-labeled cells after transplantation in vivo. Human amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) have the capacity for neuron-like differentiation that could be used to cure central nervous system (CNS) diseases. The study investigated the impacts of cytotoxicity of SPIONs on neuron-like differentiation of hAM-dMSCs in both single (1×) and multiple (4×) SPI-ONs-labeled methods. hAM-dMSCs could be efficiently labeled at safe concentrations of SPIONs (≤14 μg/ml) without significantly affecting their viability (>80% after a MTT assay), special surface antigens (CD29, CD44, CD90, CD105 through flow cytometry), and neuron-like differentiation (nestin and neuron-specific enolase through immunocytochemistry and reverse transcription polymerase chain reaction). Compared with multiple (4×) SPION-labeled methods, a single (1×) SPION-labeled method avoided multiple SPION-labeled hAM-dMSCs and minimized the impact of cytotoxicity of SPIONs on neuron-like differentiation of hAM-dMSCs. Under safe concentrations of SPIONs, a single (1×) SPION-labeled method provided appropriate viability for SPIONs-labeled hAM-dMSCs and facilitated the MRI evaluation of hAM-dMSCs after transplantation.

Original languageEnglish (US)
Pages (from-to)331-341
Number of pages11
JournalMolecular and Cellular Biochemistry
Volume357
Issue number1-2
DOIs
StatePublished - Nov 2011
Externally publishedYes

Fingerprint

Amnion
Stem cells
Mesenchymal Stromal Cells
Nanoparticles
Neurons
Membranes
Magnetic resonance
Cytotoxicity
In Vitro Techniques
ferric oxide
Magnetic Resonance Imaging
Mesenchymal Stem Cell Transplantation
Imaging techniques
Nestin
Flow cytometry
Phosphopyruvate Hydratase
Polymerase chain reaction
Central Nervous System Diseases
Cell Transplantation
Neurology

Keywords

  • Amniotic membrane
  • Mesenchymal stem cells
  • Neuron-like differentiation
  • Superparamagnetic iron oxide nanoparticles

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles : The effect on neuron-like differentiation in vitro. / Zeng, Guangwei; Wang, Gongping; Guan, Fangxia; Chang, Keliang; Jiao, Hongliang; Gao, Wanqin; Xi, Shoumin; Yang, Bo.

In: Molecular and Cellular Biochemistry, Vol. 357, No. 1-2, 11.2011, p. 331-341.

Research output: Contribution to journalArticle

Zeng, Guangwei ; Wang, Gongping ; Guan, Fangxia ; Chang, Keliang ; Jiao, Hongliang ; Gao, Wanqin ; Xi, Shoumin ; Yang, Bo. / Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles : The effect on neuron-like differentiation in vitro. In: Molecular and Cellular Biochemistry. 2011 ; Vol. 357, No. 1-2. pp. 331-341.
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