Human amniotic membrane derived-mesenchymal stem cells induce C6 glioma apoptosis in vivo through the Bcl-2/caspase pathways

Hongliang Jiao, Fangxia Guan, Bo Yang, Jianbin Li, Laijun Song, Xiang Hu, Ying Du

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

High-grade gliomas are difficult to treat. We examined the therapeutic effect of intratumoral administration of human amniotic membrane derived-mesenchymal stem cells (hAMCs) on the growth of gliomas. Tumor volume of the control group was 1632 ± 316 mm 3 on day 30, and the group treated with a single intratumoral dose of hAMCs had a tumor volume of 1128 ± 269 mm 3 (P < 0.05). Thus, administration of hAMCs significantly reduced tumor size. In rat glioma tissues treated with single and multiple dosages of hAMCs, there was a reduction in tumor volume of approximately 30.9 and 49.5%, respectively. We further evaluated the glioma tissue using Western blotting analysis and observed that the expression of Bax, caspase-8 and caspase-3 were greatly increased and the expression of Bcl-2 was greatly decreased in tumors treated with hAMCs. Sections of nude mice treated with hAMCs clearly showed the presence of an increase in apoptotic cells. The data collected herein confirms for the first time that hAMCs can inhibit C6 glioma growth and induce apoptosis of C6 gliomas in vivo. This demonstrates that hAMCs are a potential new therapeutic agent for the treatment of gliomas.

Original languageEnglish (US)
Pages (from-to)467-473
Number of pages7
JournalMolecular Biology Reports
Volume39
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

Fingerprint

Caspase 2
Amnion
Mesenchymal Stromal Cells
Glioma
Apoptosis
Tumor Burden
Caspase 8
Therapeutic Uses
Growth
Nude Mice
Caspase 3
Neoplasms
Western Blotting
Control Groups

Keywords

  • Amniotic membrane
  • Glioma
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Human amniotic membrane derived-mesenchymal stem cells induce C6 glioma apoptosis in vivo through the Bcl-2/caspase pathways. / Jiao, Hongliang; Guan, Fangxia; Yang, Bo; Li, Jianbin; Song, Laijun; Hu, Xiang; Du, Ying.

In: Molecular Biology Reports, Vol. 39, No. 1, 01.2012, p. 467-473.

Research output: Contribution to journalArticle

Jiao, Hongliang ; Guan, Fangxia ; Yang, Bo ; Li, Jianbin ; Song, Laijun ; Hu, Xiang ; Du, Ying. / Human amniotic membrane derived-mesenchymal stem cells induce C6 glioma apoptosis in vivo through the Bcl-2/caspase pathways. In: Molecular Biology Reports. 2012 ; Vol. 39, No. 1. pp. 467-473.
@article{f6c85233a8e8488ab17bddbc6fa515f0,
title = "Human amniotic membrane derived-mesenchymal stem cells induce C6 glioma apoptosis in vivo through the Bcl-2/caspase pathways",
abstract = "High-grade gliomas are difficult to treat. We examined the therapeutic effect of intratumoral administration of human amniotic membrane derived-mesenchymal stem cells (hAMCs) on the growth of gliomas. Tumor volume of the control group was 1632 ± 316 mm 3 on day 30, and the group treated with a single intratumoral dose of hAMCs had a tumor volume of 1128 ± 269 mm 3 (P < 0.05). Thus, administration of hAMCs significantly reduced tumor size. In rat glioma tissues treated with single and multiple dosages of hAMCs, there was a reduction in tumor volume of approximately 30.9 and 49.5{\%}, respectively. We further evaluated the glioma tissue using Western blotting analysis and observed that the expression of Bax, caspase-8 and caspase-3 were greatly increased and the expression of Bcl-2 was greatly decreased in tumors treated with hAMCs. Sections of nude mice treated with hAMCs clearly showed the presence of an increase in apoptotic cells. The data collected herein confirms for the first time that hAMCs can inhibit C6 glioma growth and induce apoptosis of C6 gliomas in vivo. This demonstrates that hAMCs are a potential new therapeutic agent for the treatment of gliomas.",
keywords = "Amniotic membrane, Glioma, Mesenchymal stem cells",
author = "Hongliang Jiao and Fangxia Guan and Bo Yang and Jianbin Li and Laijun Song and Xiang Hu and Ying Du",
year = "2012",
month = "1",
doi = "10.1007/s11033-011-0760-z",
language = "English (US)",
volume = "39",
pages = "467--473",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Human amniotic membrane derived-mesenchymal stem cells induce C6 glioma apoptosis in vivo through the Bcl-2/caspase pathways

AU - Jiao, Hongliang

AU - Guan, Fangxia

AU - Yang, Bo

AU - Li, Jianbin

AU - Song, Laijun

AU - Hu, Xiang

AU - Du, Ying

PY - 2012/1

Y1 - 2012/1

N2 - High-grade gliomas are difficult to treat. We examined the therapeutic effect of intratumoral administration of human amniotic membrane derived-mesenchymal stem cells (hAMCs) on the growth of gliomas. Tumor volume of the control group was 1632 ± 316 mm 3 on day 30, and the group treated with a single intratumoral dose of hAMCs had a tumor volume of 1128 ± 269 mm 3 (P < 0.05). Thus, administration of hAMCs significantly reduced tumor size. In rat glioma tissues treated with single and multiple dosages of hAMCs, there was a reduction in tumor volume of approximately 30.9 and 49.5%, respectively. We further evaluated the glioma tissue using Western blotting analysis and observed that the expression of Bax, caspase-8 and caspase-3 were greatly increased and the expression of Bcl-2 was greatly decreased in tumors treated with hAMCs. Sections of nude mice treated with hAMCs clearly showed the presence of an increase in apoptotic cells. The data collected herein confirms for the first time that hAMCs can inhibit C6 glioma growth and induce apoptosis of C6 gliomas in vivo. This demonstrates that hAMCs are a potential new therapeutic agent for the treatment of gliomas.

AB - High-grade gliomas are difficult to treat. We examined the therapeutic effect of intratumoral administration of human amniotic membrane derived-mesenchymal stem cells (hAMCs) on the growth of gliomas. Tumor volume of the control group was 1632 ± 316 mm 3 on day 30, and the group treated with a single intratumoral dose of hAMCs had a tumor volume of 1128 ± 269 mm 3 (P < 0.05). Thus, administration of hAMCs significantly reduced tumor size. In rat glioma tissues treated with single and multiple dosages of hAMCs, there was a reduction in tumor volume of approximately 30.9 and 49.5%, respectively. We further evaluated the glioma tissue using Western blotting analysis and observed that the expression of Bax, caspase-8 and caspase-3 were greatly increased and the expression of Bcl-2 was greatly decreased in tumors treated with hAMCs. Sections of nude mice treated with hAMCs clearly showed the presence of an increase in apoptotic cells. The data collected herein confirms for the first time that hAMCs can inhibit C6 glioma growth and induce apoptosis of C6 gliomas in vivo. This demonstrates that hAMCs are a potential new therapeutic agent for the treatment of gliomas.

KW - Amniotic membrane

KW - Glioma

KW - Mesenchymal stem cells

UR - http://www.scopus.com/inward/record.url?scp=84855187339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855187339&partnerID=8YFLogxK

U2 - 10.1007/s11033-011-0760-z

DO - 10.1007/s11033-011-0760-z

M3 - Article

VL - 39

SP - 467

EP - 473

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 1

ER -