Human amniotic membrane-derived mesenchymal stem cells combined with nerve growth factor and biologic fibrin glue transplantation in the treatment of bran injury in rats

Hui Wang, Fangxia Guan, Bo Yang, Yi Jun Qi, Lai Jun Song, Ying Du, Xiang Hu, Wei Hu, Hong Liang Jiao, Yuan Li

Research output: Contribution to journalArticle

Abstract

Background: Previous studies are on in vitro culture of mesenchymal stem cell differentiation. Few studies on mesenchymal stem cell differentiation in vivo or effects of cofactor on their differentiation. Objective: To study the amniotic membrane-derived mesenchymal stem cell (AD-MSC) transplantation on behavior, spatial learning and memory in traumatic brain injury rats, and the abilities to nerve growth factor (NGF), biologic fibrin glue (BFG) in AD-MSC transplantation. Design, Time and Setting: The cytology in vivo controlled study was performed at the Medical College of Zhengzhou University from July 2006 to January 2007. Materials: The placenta from healthy normal full-term fetus was obtained from Department of Gynaecology and Obstetrics, First Affiliated Hospital, Zhengzhou University. A total of 90 Wistar rats were equally and randomly assigned into a sham operation group, a model control group, a cell transplantation group, a cell + NGF group, a cell + BFG group. Methods: Fetal amniotic membrane was harvested from the placenta by blunt dissection under a sterile condition, made into monoplast suspension by trypsinization, and purified by adherece. At the third passage, AD-MSCs were used. Rats in the sham operation group underwent perforation. Rats in other groups were established into models of traumatic brain injury using free-failing epidural impact method. At 1 day following model induction, 40 μ L saline was infused into injury sites in the model control group. An equal volume of saline containing 1×10 7 AD-MSCs were injected into rats in the cell transplantation group. Rats in the cell + NGF group were injected with 1×10 7 AD-MSCs and 0.5 μ g NGF for successively for 2 weeks. Rats in the cell + BFG group were infused with AD-MSCs and 40 μ L BFG. Rats in the sham operation group did not receive cell transplantation. Main Outcome Measures: Ethology score; Latency was measured using Morris water maze test. Neuron specific enolase and glial fibrillary acidic protein expression was observed by immunohistochemistry. Results: At 7 days after transplantation, behavior score was significantly higher in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The increase was significantly lower in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=155.322, P < 0.05). At 3 weeks after the transplantation, the latency was significantly shorter in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The shortened range was significantly smaller in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=22.678, P < 0.05). At 4 weeks following transplantation, AD-MSCs could differentiate into nerve cell in damage brain tissue, and express neural specific enolase and glial fibrillary acidic protein following combined transplantation of NGF and BFG (F=705.406, F=424.884, P < 0.05). Conclusion: AD-MSCs can improve behavior and spacial learning and memory abilities, express neural specific enolase and glial fibrillary acidic protein following differentiation. NGF and BFG may enhance efficacy of transplantation.

Original languageEnglish (US)
Pages (from-to)10005-10009
Number of pages5
JournalJournal of Clinical Rehabilitative Tissue Engineering Research
Volume12
Issue number51
StatePublished - Dec 16 2008
Externally publishedYes

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Fibrin Tissue Adhesive
Amnion
Glues
Nerve Growth Factor
Stem cells
Mesenchymal Stromal Cells
Rats
Transplantation
Neurons
Membranes
Cell Transplantation
Wounds and Injuries
Phosphopyruvate Hydratase
Glial Fibrillary Acidic Protein
Therapeutics
Brain
Mesenchymal Stem Cell Transplantation
Control Groups
Proteins
Aptitude

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biomedical Engineering
  • Transplantation

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Human amniotic membrane-derived mesenchymal stem cells combined with nerve growth factor and biologic fibrin glue transplantation in the treatment of bran injury in rats. / Wang, Hui; Guan, Fangxia; Yang, Bo; Qi, Yi Jun; Song, Lai Jun; Du, Ying; Hu, Xiang; Hu, Wei; Jiao, Hong Liang; Li, Yuan.

In: Journal of Clinical Rehabilitative Tissue Engineering Research, Vol. 12, No. 51, 16.12.2008, p. 10005-10009.

Research output: Contribution to journalArticle

Wang, Hui ; Guan, Fangxia ; Yang, Bo ; Qi, Yi Jun ; Song, Lai Jun ; Du, Ying ; Hu, Xiang ; Hu, Wei ; Jiao, Hong Liang ; Li, Yuan. / Human amniotic membrane-derived mesenchymal stem cells combined with nerve growth factor and biologic fibrin glue transplantation in the treatment of bran injury in rats. In: Journal of Clinical Rehabilitative Tissue Engineering Research. 2008 ; Vol. 12, No. 51. pp. 10005-10009.
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abstract = "Background: Previous studies are on in vitro culture of mesenchymal stem cell differentiation. Few studies on mesenchymal stem cell differentiation in vivo or effects of cofactor on their differentiation. Objective: To study the amniotic membrane-derived mesenchymal stem cell (AD-MSC) transplantation on behavior, spatial learning and memory in traumatic brain injury rats, and the abilities to nerve growth factor (NGF), biologic fibrin glue (BFG) in AD-MSC transplantation. Design, Time and Setting: The cytology in vivo controlled study was performed at the Medical College of Zhengzhou University from July 2006 to January 2007. Materials: The placenta from healthy normal full-term fetus was obtained from Department of Gynaecology and Obstetrics, First Affiliated Hospital, Zhengzhou University. A total of 90 Wistar rats were equally and randomly assigned into a sham operation group, a model control group, a cell transplantation group, a cell + NGF group, a cell + BFG group. Methods: Fetal amniotic membrane was harvested from the placenta by blunt dissection under a sterile condition, made into monoplast suspension by trypsinization, and purified by adherece. At the third passage, AD-MSCs were used. Rats in the sham operation group underwent perforation. Rats in other groups were established into models of traumatic brain injury using free-failing epidural impact method. At 1 day following model induction, 40 μ L saline was infused into injury sites in the model control group. An equal volume of saline containing 1×10 7 AD-MSCs were injected into rats in the cell transplantation group. Rats in the cell + NGF group were injected with 1×10 7 AD-MSCs and 0.5 μ g NGF for successively for 2 weeks. Rats in the cell + BFG group were infused with AD-MSCs and 40 μ L BFG. Rats in the sham operation group did not receive cell transplantation. Main Outcome Measures: Ethology score; Latency was measured using Morris water maze test. Neuron specific enolase and glial fibrillary acidic protein expression was observed by immunohistochemistry. Results: At 7 days after transplantation, behavior score was significantly higher in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The increase was significantly lower in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=155.322, P < 0.05). At 3 weeks after the transplantation, the latency was significantly shorter in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The shortened range was significantly smaller in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=22.678, P < 0.05). At 4 weeks following transplantation, AD-MSCs could differentiate into nerve cell in damage brain tissue, and express neural specific enolase and glial fibrillary acidic protein following combined transplantation of NGF and BFG (F=705.406, F=424.884, P < 0.05). Conclusion: AD-MSCs can improve behavior and spacial learning and memory abilities, express neural specific enolase and glial fibrillary acidic protein following differentiation. NGF and BFG may enhance efficacy of transplantation.",
author = "Hui Wang and Fangxia Guan and Bo Yang and Qi, {Yi Jun} and Song, {Lai Jun} and Ying Du and Xiang Hu and Wei Hu and Jiao, {Hong Liang} and Yuan Li",
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month = "12",
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volume = "12",
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TY - JOUR

T1 - Human amniotic membrane-derived mesenchymal stem cells combined with nerve growth factor and biologic fibrin glue transplantation in the treatment of bran injury in rats

AU - Wang, Hui

AU - Guan, Fangxia

AU - Yang, Bo

AU - Qi, Yi Jun

AU - Song, Lai Jun

AU - Du, Ying

AU - Hu, Xiang

AU - Hu, Wei

AU - Jiao, Hong Liang

AU - Li, Yuan

PY - 2008/12/16

Y1 - 2008/12/16

N2 - Background: Previous studies are on in vitro culture of mesenchymal stem cell differentiation. Few studies on mesenchymal stem cell differentiation in vivo or effects of cofactor on their differentiation. Objective: To study the amniotic membrane-derived mesenchymal stem cell (AD-MSC) transplantation on behavior, spatial learning and memory in traumatic brain injury rats, and the abilities to nerve growth factor (NGF), biologic fibrin glue (BFG) in AD-MSC transplantation. Design, Time and Setting: The cytology in vivo controlled study was performed at the Medical College of Zhengzhou University from July 2006 to January 2007. Materials: The placenta from healthy normal full-term fetus was obtained from Department of Gynaecology and Obstetrics, First Affiliated Hospital, Zhengzhou University. A total of 90 Wistar rats were equally and randomly assigned into a sham operation group, a model control group, a cell transplantation group, a cell + NGF group, a cell + BFG group. Methods: Fetal amniotic membrane was harvested from the placenta by blunt dissection under a sterile condition, made into monoplast suspension by trypsinization, and purified by adherece. At the third passage, AD-MSCs were used. Rats in the sham operation group underwent perforation. Rats in other groups were established into models of traumatic brain injury using free-failing epidural impact method. At 1 day following model induction, 40 μ L saline was infused into injury sites in the model control group. An equal volume of saline containing 1×10 7 AD-MSCs were injected into rats in the cell transplantation group. Rats in the cell + NGF group were injected with 1×10 7 AD-MSCs and 0.5 μ g NGF for successively for 2 weeks. Rats in the cell + BFG group were infused with AD-MSCs and 40 μ L BFG. Rats in the sham operation group did not receive cell transplantation. Main Outcome Measures: Ethology score; Latency was measured using Morris water maze test. Neuron specific enolase and glial fibrillary acidic protein expression was observed by immunohistochemistry. Results: At 7 days after transplantation, behavior score was significantly higher in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The increase was significantly lower in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=155.322, P < 0.05). At 3 weeks after the transplantation, the latency was significantly shorter in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The shortened range was significantly smaller in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=22.678, P < 0.05). At 4 weeks following transplantation, AD-MSCs could differentiate into nerve cell in damage brain tissue, and express neural specific enolase and glial fibrillary acidic protein following combined transplantation of NGF and BFG (F=705.406, F=424.884, P < 0.05). Conclusion: AD-MSCs can improve behavior and spacial learning and memory abilities, express neural specific enolase and glial fibrillary acidic protein following differentiation. NGF and BFG may enhance efficacy of transplantation.

AB - Background: Previous studies are on in vitro culture of mesenchymal stem cell differentiation. Few studies on mesenchymal stem cell differentiation in vivo or effects of cofactor on their differentiation. Objective: To study the amniotic membrane-derived mesenchymal stem cell (AD-MSC) transplantation on behavior, spatial learning and memory in traumatic brain injury rats, and the abilities to nerve growth factor (NGF), biologic fibrin glue (BFG) in AD-MSC transplantation. Design, Time and Setting: The cytology in vivo controlled study was performed at the Medical College of Zhengzhou University from July 2006 to January 2007. Materials: The placenta from healthy normal full-term fetus was obtained from Department of Gynaecology and Obstetrics, First Affiliated Hospital, Zhengzhou University. A total of 90 Wistar rats were equally and randomly assigned into a sham operation group, a model control group, a cell transplantation group, a cell + NGF group, a cell + BFG group. Methods: Fetal amniotic membrane was harvested from the placenta by blunt dissection under a sterile condition, made into monoplast suspension by trypsinization, and purified by adherece. At the third passage, AD-MSCs were used. Rats in the sham operation group underwent perforation. Rats in other groups were established into models of traumatic brain injury using free-failing epidural impact method. At 1 day following model induction, 40 μ L saline was infused into injury sites in the model control group. An equal volume of saline containing 1×10 7 AD-MSCs were injected into rats in the cell transplantation group. Rats in the cell + NGF group were injected with 1×10 7 AD-MSCs and 0.5 μ g NGF for successively for 2 weeks. Rats in the cell + BFG group were infused with AD-MSCs and 40 μ L BFG. Rats in the sham operation group did not receive cell transplantation. Main Outcome Measures: Ethology score; Latency was measured using Morris water maze test. Neuron specific enolase and glial fibrillary acidic protein expression was observed by immunohistochemistry. Results: At 7 days after transplantation, behavior score was significantly higher in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The increase was significantly lower in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=155.322, P < 0.05). At 3 weeks after the transplantation, the latency was significantly shorter in the cell transplantation group, cell + NGF group, cell + BFG group compared with the model control group. The shortened range was significantly smaller in the cell transplantation group compared with the cell + NGF group and cell + BFG group (F=22.678, P < 0.05). At 4 weeks following transplantation, AD-MSCs could differentiate into nerve cell in damage brain tissue, and express neural specific enolase and glial fibrillary acidic protein following combined transplantation of NGF and BFG (F=705.406, F=424.884, P < 0.05). Conclusion: AD-MSCs can improve behavior and spacial learning and memory abilities, express neural specific enolase and glial fibrillary acidic protein following differentiation. NGF and BFG may enhance efficacy of transplantation.

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