Human α1(III) and α2(V) procollagen genes are located on the long arm of chromosome 2

B. S. Emanuel, L. A. Cannizzaro, J. M. Seyer, J. C. Myers

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The multigene procollagen family encodes probably >20 genetically distinct but structurally related polypeptide chains. Recent characterization of human procollagen clones has allowed determination of functional domains within the proteins, genomic organization, and chromosomal location. Previously, we assigned the coordinately expressed type I genes (α1 and α2) to chromosomes 17 and 7, respectively, and now other investigators have mapped the type II gene to chromosome 12. Recently, we isolated cDNA clones encoding the fourth interstitial procollagen, type III, and the α2 chain of the type V cytoskeletal components. To determine whether these genes were clustered with α1(I), α2(I), or α1(II) or were further dispersed in the genome, in situ hybridization of the α1(III) and α2(V) probes to metaphase chromosomes was carried out. Here we report a fourth autosome with procollagen gene loci but the first cytological evidence for linkage. By using normal and translocated cell lines, our results show that both the α1(III) and α2(V) procollagen genes map to the q24.3→q31 region of chromosome 2.

Original languageEnglish (US)
Pages (from-to)3385-3389
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number10
StatePublished - 1985
Externally publishedYes

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Procollagen
Chromosomes, Human, Pair 2
Genes
Clone Cells
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 7
Collagen Type II
Metaphase
Multigene Family
In Situ Hybridization
Complementary DNA
Chromosomes
Research Personnel
Genome
Cell Line
Peptides

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Human α1(III) and α2(V) procollagen genes are located on the long arm of chromosome 2. / Emanuel, B. S.; Cannizzaro, L. A.; Seyer, J. M.; Myers, J. C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 10, 1985, p. 3385-3389.

Research output: Contribution to journalArticle

Emanuel, B. S. ; Cannizzaro, L. A. ; Seyer, J. M. ; Myers, J. C. / Human α1(III) and α2(V) procollagen genes are located on the long arm of chromosome 2. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 10. pp. 3385-3389.
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