Human α-1-acid glycoprotein inhibits TNF production in the CNS of rabbits with meningitis

A report of preliminary observations

David F. Moore, Liana Tsenova, Laura Corral, Victoria H. Freedman, Gilla Kaplan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bacterial meningitis is accompanied by an acute inflammatory response which may be exacerbated by antibiotic treatment and subsequent killing of bacteria. Bacterial cell products induce the release of cytokines including TNFα, which contribute to the inflammatory process. Alpha-1-acid glycoprotein (AAG), an acute phase reactant, is elevated during inflammation. To test whether AAG has anti-inflammatory activity we examined its effect on lipopolysaccharide-stimulated human peripheral blood mononuclear cells. Treatment of the cells with AAG in vitro resulted in reduced TNFα production. To test the effects of the molecule in vivo, AAG was administered intrathecally to rabbits with Haemophilus influenzae B. lysate induced meningitis. Human AAG reduced TNFα production and leukocytosis in the cerebrospinal fluid. Histopathology of the leptomeninges showed markedly attenuated inflammation. These results indicate that AAG can reduce inflammation in rabbits with experimental meningitis and that the effect may be directly on TNFα production by stimulated mononuclear leukocytes.

Original languageEnglish (US)
Pages (from-to)265-275
Number of pages11
JournalInflammation
Volume24
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Orosomucoid
Meningitis
Glycoproteins
Rabbits
Acids
Inflammation
Mononuclear Leukocytes
Bacterial Meningitides
Acute-Phase Proteins
Leukocytosis
Haemophilus influenzae
Lipopolysaccharides
Cerebrospinal Fluid
Blood Cells
Anti-Inflammatory Agents
Cytokines
Anti-Bacterial Agents
Bacteria

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Medicine(all)

Cite this

Human α-1-acid glycoprotein inhibits TNF production in the CNS of rabbits with meningitis : A report of preliminary observations. / Moore, David F.; Tsenova, Liana; Corral, Laura; Freedman, Victoria H.; Kaplan, Gilla.

In: Inflammation, Vol. 24, No. 3, 2000, p. 265-275.

Research output: Contribution to journalArticle

Moore, David F. ; Tsenova, Liana ; Corral, Laura ; Freedman, Victoria H. ; Kaplan, Gilla. / Human α-1-acid glycoprotein inhibits TNF production in the CNS of rabbits with meningitis : A report of preliminary observations. In: Inflammation. 2000 ; Vol. 24, No. 3. pp. 265-275.
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