HPV infection and cervical screening in socially isolated indigenous women inhabitants of the amazonian rainforest

Allex Jardim Fonseca, Daniela Taeko, Thiciane Araújo Chaves, Lucia Dayanny Da Costa Amorim, Raisa Saron Wanderley Murari, Angélica Espinosa Miranda, Zigui Chen, Robert D. Burk, Luiz Carlos Lima Ferreira

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective Indigenous women from the Amazon regions have some of the highest rates of cervical cancer in the world. This study evaluated cervical cytology and human papillomavirus (HPV) in native women that differ by lifestyle and interaction with western society. Yanomami women are isolated deep in the Amazon with a hunter/gatherer lifestyle. Macuxi and Wapishana women live in proximity to western society. Methods To select a representative group of women from each district, random cluster sampling was used, considering each registered village as a cluster. Cervical samples were collected for cytology and HPV detection and typing by PCR amplification and next generation sequencing. The study was approved by the National IRB and by tribal leaders. Results 664 native women were enrolled from 13 indigenous villages (76% participation rate). Yanomami women had higher rates of abnormal cytology (5.1% vs. 1.8%, p = 0.04) and prevalent HR-HPV (34.1% vs. 19.2%, p<0.001). Yanomami women >35y of age were significantly more likely to have HR-HPV, whereas women 35y did not significantly differ between groups. Prevalence of HPV was significantly different amongst geographically clustered Yanomami women (p<0.004). The most prevalent HPV types in the entire group were HPV31 (8.7%), HPV16 (5.9%) and HPV18 (4.4%). Conclusion Isolated endogenous Yanomami women were more likely to be HPV+ and rates increased with age. Study of HPV in isolated hunter-gather peoples suggests that long-term persistence is a characteristic of prehistoric humans and patterns reflecting decreased prevalence with age in western society represents recent change. These studies have implications for cervical cancer prevention and viral-host relationships.

Original languageEnglish (US)
Article numbere0133635
JournalPLoS One
Volume10
Issue number7
DOIs
StatePublished - Jul 24 2015

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Cytology
Papillomavirus Infections
Papillomaviridae
rain forests
Screening
screening
infection
Amplification
cell biology
Cell Biology
uterine cervical neoplasms
Sampling
Population Groups
Uterine Cervical Neoplasms
lifestyle
Life Style
villages
Rainforest
Research Ethics Committees
Amazonia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fonseca, A. J., Taeko, D., Chaves, T. A., Da Costa Amorim, L. D., Murari, R. S. W., Miranda, A. E., ... Ferreira, L. C. L. (2015). HPV infection and cervical screening in socially isolated indigenous women inhabitants of the amazonian rainforest. PLoS One, 10(7), [e0133635]. https://doi.org/10.1371/journal.pone.0133635

HPV infection and cervical screening in socially isolated indigenous women inhabitants of the amazonian rainforest. / Fonseca, Allex Jardim; Taeko, Daniela; Chaves, Thiciane Araújo; Da Costa Amorim, Lucia Dayanny; Murari, Raisa Saron Wanderley; Miranda, Angélica Espinosa; Chen, Zigui; Burk, Robert D.; Ferreira, Luiz Carlos Lima.

In: PLoS One, Vol. 10, No. 7, e0133635, 24.07.2015.

Research output: Contribution to journalArticle

Fonseca, AJ, Taeko, D, Chaves, TA, Da Costa Amorim, LD, Murari, RSW, Miranda, AE, Chen, Z, Burk, RD & Ferreira, LCL 2015, 'HPV infection and cervical screening in socially isolated indigenous women inhabitants of the amazonian rainforest', PLoS One, vol. 10, no. 7, e0133635. https://doi.org/10.1371/journal.pone.0133635
Fonseca AJ, Taeko D, Chaves TA, Da Costa Amorim LD, Murari RSW, Miranda AE et al. HPV infection and cervical screening in socially isolated indigenous women inhabitants of the amazonian rainforest. PLoS One. 2015 Jul 24;10(7). e0133635. https://doi.org/10.1371/journal.pone.0133635
Fonseca, Allex Jardim ; Taeko, Daniela ; Chaves, Thiciane Araújo ; Da Costa Amorim, Lucia Dayanny ; Murari, Raisa Saron Wanderley ; Miranda, Angélica Espinosa ; Chen, Zigui ; Burk, Robert D. ; Ferreira, Luiz Carlos Lima. / HPV infection and cervical screening in socially isolated indigenous women inhabitants of the amazonian rainforest. In: PLoS One. 2015 ; Vol. 10, No. 7.
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abstract = "Objective Indigenous women from the Amazon regions have some of the highest rates of cervical cancer in the world. This study evaluated cervical cytology and human papillomavirus (HPV) in native women that differ by lifestyle and interaction with western society. Yanomami women are isolated deep in the Amazon with a hunter/gatherer lifestyle. Macuxi and Wapishana women live in proximity to western society. Methods To select a representative group of women from each district, random cluster sampling was used, considering each registered village as a cluster. Cervical samples were collected for cytology and HPV detection and typing by PCR amplification and next generation sequencing. The study was approved by the National IRB and by tribal leaders. Results 664 native women were enrolled from 13 indigenous villages (76{\%} participation rate). Yanomami women had higher rates of abnormal cytology (5.1{\%} vs. 1.8{\%}, p = 0.04) and prevalent HR-HPV (34.1{\%} vs. 19.2{\%}, p<0.001). Yanomami women >35y of age were significantly more likely to have HR-HPV, whereas women 35y did not significantly differ between groups. Prevalence of HPV was significantly different amongst geographically clustered Yanomami women (p<0.004). The most prevalent HPV types in the entire group were HPV31 (8.7{\%}), HPV16 (5.9{\%}) and HPV18 (4.4{\%}). Conclusion Isolated endogenous Yanomami women were more likely to be HPV+ and rates increased with age. Study of HPV in isolated hunter-gather peoples suggests that long-term persistence is a characteristic of prehistoric humans and patterns reflecting decreased prevalence with age in western society represents recent change. These studies have implications for cervical cancer prevention and viral-host relationships.",
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N2 - Objective Indigenous women from the Amazon regions have some of the highest rates of cervical cancer in the world. This study evaluated cervical cytology and human papillomavirus (HPV) in native women that differ by lifestyle and interaction with western society. Yanomami women are isolated deep in the Amazon with a hunter/gatherer lifestyle. Macuxi and Wapishana women live in proximity to western society. Methods To select a representative group of women from each district, random cluster sampling was used, considering each registered village as a cluster. Cervical samples were collected for cytology and HPV detection and typing by PCR amplification and next generation sequencing. The study was approved by the National IRB and by tribal leaders. Results 664 native women were enrolled from 13 indigenous villages (76% participation rate). Yanomami women had higher rates of abnormal cytology (5.1% vs. 1.8%, p = 0.04) and prevalent HR-HPV (34.1% vs. 19.2%, p<0.001). Yanomami women >35y of age were significantly more likely to have HR-HPV, whereas women 35y did not significantly differ between groups. Prevalence of HPV was significantly different amongst geographically clustered Yanomami women (p<0.004). The most prevalent HPV types in the entire group were HPV31 (8.7%), HPV16 (5.9%) and HPV18 (4.4%). Conclusion Isolated endogenous Yanomami women were more likely to be HPV+ and rates increased with age. Study of HPV in isolated hunter-gather peoples suggests that long-term persistence is a characteristic of prehistoric humans and patterns reflecting decreased prevalence with age in western society represents recent change. These studies have implications for cervical cancer prevention and viral-host relationships.

AB - Objective Indigenous women from the Amazon regions have some of the highest rates of cervical cancer in the world. This study evaluated cervical cytology and human papillomavirus (HPV) in native women that differ by lifestyle and interaction with western society. Yanomami women are isolated deep in the Amazon with a hunter/gatherer lifestyle. Macuxi and Wapishana women live in proximity to western society. Methods To select a representative group of women from each district, random cluster sampling was used, considering each registered village as a cluster. Cervical samples were collected for cytology and HPV detection and typing by PCR amplification and next generation sequencing. The study was approved by the National IRB and by tribal leaders. Results 664 native women were enrolled from 13 indigenous villages (76% participation rate). Yanomami women had higher rates of abnormal cytology (5.1% vs. 1.8%, p = 0.04) and prevalent HR-HPV (34.1% vs. 19.2%, p<0.001). Yanomami women >35y of age were significantly more likely to have HR-HPV, whereas women 35y did not significantly differ between groups. Prevalence of HPV was significantly different amongst geographically clustered Yanomami women (p<0.004). The most prevalent HPV types in the entire group were HPV31 (8.7%), HPV16 (5.9%) and HPV18 (4.4%). Conclusion Isolated endogenous Yanomami women were more likely to be HPV+ and rates increased with age. Study of HPV in isolated hunter-gather peoples suggests that long-term persistence is a characteristic of prehistoric humans and patterns reflecting decreased prevalence with age in western society represents recent change. These studies have implications for cervical cancer prevention and viral-host relationships.

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