HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood

Ligia A. Pinto, Philip E. Castle, Richard B. Roden, Clayton D. Harro, Douglas R. Lowy, John T. Schiller, Dora Wallace, Marcus Williams, William Kopp, Ian H. Frazer, Jay A. Berzofsky, Allan Hildesheim

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n = 20) or placebo (n = 4) before and after vaccination. 11 cytokines were measured: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IFN-γ, TNF-α, and GM-CSF using multiplex bead arrays. Cytokine profiles from WB samples clearly discriminated between vaccine and placebo recipients and between pre and post-vaccination responses. Significant increases in Th1, Th2 and inflammatory cytokines were observed in WB assays following vaccination. Results from WB assays were compared against parallel PBMC-based assays in a subset of patients. Differences between whole blood assay and PBMC were observed, with the highest levels of induction found for WB for several cytokines. Our results indicate that multiplex assays for cytokine profiling in WB are an efficient tool for assessing broad spectrum, innate and adaptive immune responses to vaccines and identifying immunologic correlates of protection in efficacy studies.

Original languageEnglish (US)
Pages (from-to)3555-3564
Number of pages10
JournalVaccine
Volume23
Issue number27
DOIs
StatePublished - May 20 2005
Externally publishedYes

Fingerprint

virus-like particle vaccines
Human papillomavirus 16
cytokines
Vaccines
Cytokines
blood
Vaccination
assays
vaccination
vaccines
mononuclear leukocytes
placebos
Placebos
Interleukin-5
interleukin-5
Adaptive Immunity
Interleukin-12
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Interleukin-1

Keywords

  • Cytokines
  • HPV-16 L1 VLP vaccine
  • Whole blood

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Pinto, L. A., Castle, P. E., Roden, R. B., Harro, C. D., Lowy, D. R., Schiller, J. T., ... Hildesheim, A. (2005). HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood. Vaccine, 23(27), 3555-3564. https://doi.org/10.1016/j.vaccine.2005.01.146

HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood. / Pinto, Ligia A.; Castle, Philip E.; Roden, Richard B.; Harro, Clayton D.; Lowy, Douglas R.; Schiller, John T.; Wallace, Dora; Williams, Marcus; Kopp, William; Frazer, Ian H.; Berzofsky, Jay A.; Hildesheim, Allan.

In: Vaccine, Vol. 23, No. 27, 20.05.2005, p. 3555-3564.

Research output: Contribution to journalArticle

Pinto, LA, Castle, PE, Roden, RB, Harro, CD, Lowy, DR, Schiller, JT, Wallace, D, Williams, M, Kopp, W, Frazer, IH, Berzofsky, JA & Hildesheim, A 2005, 'HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood', Vaccine, vol. 23, no. 27, pp. 3555-3564. https://doi.org/10.1016/j.vaccine.2005.01.146
Pinto, Ligia A. ; Castle, Philip E. ; Roden, Richard B. ; Harro, Clayton D. ; Lowy, Douglas R. ; Schiller, John T. ; Wallace, Dora ; Williams, Marcus ; Kopp, William ; Frazer, Ian H. ; Berzofsky, Jay A. ; Hildesheim, Allan. / HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood. In: Vaccine. 2005 ; Vol. 23, No. 27. pp. 3555-3564.
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