How to combine ChIP with qPCR

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody. However, the highly accurate quantitative dimension added by using qPCR to analyze ChIP samples significantly raises the bar in terms of experimental accuracy, appropriate controls, data analysis, and data presentation. This chapter will address these potential pitfalls by providing protocols and procedures that address the difficulties inherent in ChIP-qPCR assays.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages29-42
Number of pages14
Volume1689
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1689
ISSN (Print)1064-3745

Keywords

  • Amplicon
  • ChIP
  • ChIP-qPCR data presentation
  • Chromatin
  • Primer design
  • qPCR
  • qPCR calculations
  • Quantitative PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Asp, P. K. M. (2018). How to combine ChIP with qPCR. In Methods in Molecular Biology (Vol. 1689, pp. 29-42). (Methods in Molecular Biology; Vol. 1689). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7380-4_3