How to combine ChIP with qPCR

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody. However, the highly accurate quantitative dimension added by using qPCR to analyze ChIP samples significantly raises the bar in terms of experimental accuracy, appropriate controls, data analysis, and data presentation. This chapter will address these potential pitfalls by providing protocols and procedures that address the difficulties inherent in ChIP-qPCR assays.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages29-42
Number of pages14
Volume1689
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1689
ISSN (Print)1064-3745

Fingerprint

Chromatin Immunoprecipitation
Polymerase Chain Reaction
Computer Systems
Chromatin
Real-Time Polymerase Chain Reaction
Antibodies
Research

Keywords

  • Amplicon
  • ChIP
  • ChIP-qPCR data presentation
  • Chromatin
  • Primer design
  • qPCR
  • qPCR calculations
  • Quantitative PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Asp, P. K. M. (2018). How to combine ChIP with qPCR. In Methods in Molecular Biology (Vol. 1689, pp. 29-42). (Methods in Molecular Biology; Vol. 1689). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7380-4_3

How to combine ChIP with qPCR. / Asp, Patrik K.M.

Methods in Molecular Biology. Vol. 1689 Humana Press Inc., 2018. p. 29-42 (Methods in Molecular Biology; Vol. 1689).

Research output: Chapter in Book/Report/Conference proceedingChapter

Asp, PKM 2018, How to combine ChIP with qPCR. in Methods in Molecular Biology. vol. 1689, Methods in Molecular Biology, vol. 1689, Humana Press Inc., pp. 29-42. https://doi.org/10.1007/978-1-4939-7380-4_3
Asp PKM. How to combine ChIP with qPCR. In Methods in Molecular Biology. Vol. 1689. Humana Press Inc. 2018. p. 29-42. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7380-4_3
Asp, Patrik K.M. / How to combine ChIP with qPCR. Methods in Molecular Biology. Vol. 1689 Humana Press Inc., 2018. pp. 29-42 (Methods in Molecular Biology).
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